Proposed phase 2/ step 2 in-vitro test on basis of EN 14561 for standardised testing of the wound antiseptics PVP-iodine, chlorhexidine digluconate, polihexanide and octenidine dihydrochloride.

Autor: Schedler K; Institute of Hygiene and Environmental Medicine, University Medicine, Greifswald, Germany., Assadian O; Institute for Sin Integrity and Infection Prevention, School of Human and Health Sciences, University of Huddersfield, R1/29 Ramsden Building, Queensgate, Huddersfield, HD1 3DH, UK. Ojan.assadian@meduniwien.ac.at., Brautferger U; Institute of Hygiene and Environmental Medicine, University Medicine, Greifswald, Germany., Müller G; Institute of Hygiene and Environmental Medicine, University Medicine, Greifswald, Germany., Koburger T; Hygiene Nord GmbH, Greifswald, Germany., Classen S; Department for Vascular Surgery, Kerckhoff-Klinik GmbH, Bad Nauheim, Germany., Kramer A; Institute of Hygiene and Environmental Medicine, University Medicine, Greifswald, Germany.
Jazyk: angličtina
Zdroj: BMC infectious diseases [BMC Infect Dis] 2017 Feb 13; Vol. 17 (1), pp. 143. Date of Electronic Publication: 2017 Feb 13.
DOI: 10.1186/s12879-017-2220-4
Abstrakt: Background: Currently, there is no agreed standard for exploring the antimicrobial activity of wound antiseptics in a phase 2/ step 2 test protocol. In the present study, a standardised in-vitro test is proposed, which allows to test potential antiseptics in a more realistically simulation of conditions found in wounds as in a suspension test. Furthermore, factors potentially influencing test results such as type of materials used as test carrier or various compositions of organic soil challenge were investigated in detail.
Methods: This proposed phase 2/ step 2 test method was modified on basis of the EN 14561 by drying the microbial test suspension on a metal carrier for 1 h, overlaying the test wound antiseptic, washing-off, neutralization, and dispersion at serial dilutions at the end of the required exposure time yielded reproducible, consistent test results.
Results: The difference between the rapid onset of the antiseptic effect of PVP-I and the delayed onset especially of polihexanide was apparent. Among surface-active antimicrobial compounds, octenidine was more effective than chlorhexidine digluconate and polihexanide, with some differences depending on the test organisms. However, octenidine and PVP-I were approximately equivalent in efficiency and microbial spectrum, while polihexanide required longer exposure times or higher concentrations for a comparable antimicrobial efficacy.
Conclusion: Overall, this method allowed testing and comparing differ liquid and gel based antimicrobial compounds in a standardised setting.
Databáze: MEDLINE
Externí odkaz: