The relation between xyr1 overexpression in Trichoderma harzianum and sugarcane bagasse saccharification performance.

Autor: da Silva Delabona P; Brazilian Bioethanol Science and Technology Laboratory, CTBE, Pólo II de Alta Tecnologia, Rua Giuseppe Maximo Scolfaro 10000, Caixa Postal 6192, Campinas, SP, 13083-970, Brazil; Graduate Program of Biotechnology, Federal University of São Carlos, São Carlos, SP, 13565-905, Brazil; Molecular Biotechnology, Biochemical Technology Research Division, Institute of Chemical Engineering, TU Wien, Gumpendorferstrasse 1a, A-1060 Vienna, Austria. Electronic address: priscila.delabona@bioetanol.org.br., Rodrigues GN; Brazilian Bioethanol Science and Technology Laboratory, CTBE, Pólo II de Alta Tecnologia, Rua Giuseppe Maximo Scolfaro 10000, Caixa Postal 6192, Campinas, SP, 13083-970, Brazil., Zubieta MP; Brazilian Bioethanol Science and Technology Laboratory, CTBE, Pólo II de Alta Tecnologia, Rua Giuseppe Maximo Scolfaro 10000, Caixa Postal 6192, Campinas, SP, 13083-970, Brazil., Ramoni J; Molecular Biotechnology, Biochemical Technology Research Division, Institute of Chemical Engineering, TU Wien, Gumpendorferstrasse 1a, A-1060 Vienna, Austria., Codima CA; Brazilian Bioethanol Science and Technology Laboratory, CTBE, Pólo II de Alta Tecnologia, Rua Giuseppe Maximo Scolfaro 10000, Caixa Postal 6192, Campinas, SP, 13083-970, Brazil., Lima DJ; Brazilian Bioethanol Science and Technology Laboratory, CTBE, Pólo II de Alta Tecnologia, Rua Giuseppe Maximo Scolfaro 10000, Caixa Postal 6192, Campinas, SP, 13083-970, Brazil., Farinas CS; Graduate Program of Biotechnology, Federal University of São Carlos, São Carlos, SP, 13565-905, Brazil; Embrapa Instrumentação, Rua XV de Novembro 1452, São Carlos, SP, 13560-970, Brazil., da Cruz Pradella JG; Brazilian Bioethanol Science and Technology Laboratory, CTBE, Pólo II de Alta Tecnologia, Rua Giuseppe Maximo Scolfaro 10000, Caixa Postal 6192, Campinas, SP, 13083-970, Brazil; Graduate Program of Biotechnology, Federal University of São Carlos, São Carlos, SP, 13565-905, Brazil., Seiboth B; Molecular Biotechnology, Biochemical Technology Research Division, Institute of Chemical Engineering, TU Wien, Gumpendorferstrasse 1a, A-1060 Vienna, Austria.
Jazyk: angličtina
Zdroj: Journal of biotechnology [J Biotechnol] 2017 Mar 20; Vol. 246, pp. 24-32. Date of Electronic Publication: 2017 Feb 10.
DOI: 10.1016/j.jbiotec.2017.02.002
Abstrakt: This work investigates the influence of the positive regulator XYR1 of Trichoderma harzianum on the production of cellulolytic enzymes, using sugarcane bagasse as carbon source. Constitutive expression of xyr1 was achieved under the control of the strong Trichoderma reesei pki1 promoter. Five clones with xyr1 overexpression achieved higher xyr1 expression and greater enzymatic productivity when cultivated under submerged fermentation, hence validating the genetic construction for T. harzianum. Clone 5 presented a relative expression of xyr1 26-fold higher than the parent strain and exhibited 66, 37, and 36% higher values for filter paper activity, xylanase activity, and β-glucosidase activity, respectively, during cultivation in a stirred-tank bioreactor. The overexpression of xyr1 in T. harzianum resulted in an enzymatic complex with significantly improved performance in sugarcane bagasse saccharification, with an enhancement of 25% in the first 24h. Our results also show that constitutive overexpression of xyr1 leads to the induction of several important players in biomass degradation at early (24h) and also late (48h) timepoints of inoculation. However, we also observed that the carbon catabolite repressor CRE1 was upregulated in xyr1 overexpression mutants. These findings demonstrate the feasibility of improving cellulase production by modifying regulator expression and suggest an attractive approach for increasing total cellulase productivity in T. harzianum.
(Copyright © 2017 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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