| Autor: |
Shipulina NV, Tomilina NIu, Zhirnov GF, Lisitsyna VB, Kovalev IE, Archakov AI |
| Jazyk: |
ruština |
| Zdroj: |
Biokhimiia (Moscow, Russia) [Biokhimiia] 1989 Aug; Vol. 54 (8), pp. 1254-7. |
| Abstrakt: |
The feasibility of obtaining a conjugated benz(a)pyrene-protein antigen in the liver cytochrome P-450 system was studied. Covalent binding of benz(a)pyrene (BP) to albumin was performed with the use of liver microsomal fractions of 3-methylcholanthrene-induced rabbits. It was demonstrated that BP oxidation in liver microsomes is accompanied by covalent binding of [14C]BP to exogenous rabbit albumin. Immunization of rabbits with the obtained conjugate results in the development of a specific immune response to BP, and the appearance of specific antibodies and lymphocytes specifically binding [14C]BP in the blood. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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