Improving Proteome Coverage and Sample Recovery with Enhanced FASP (eFASP) for Quantitative Proteomic Experiments.

Autor:	Erde J; Department of Chemistry and Biochemistry, University of California-Los Angeles, Los Angeles, CA, 90095, USA., Loo RR; Department of Biological Chemistry, University of California-Los Angeles, Los Angeles, CA, 90095, USA., Loo JA; Department of Chemistry and Biochemistry, University of California-Los Angeles, Los Angeles, CA, 90095, USA. jloo@chem.ucla.edu.; Department of Biological Chemistry, University of California-Los Angeles, Los Angeles, CA, 90095, USA. jloo@chem.ucla.edu.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2017; Vol. 1550, pp. 11-18.
DOI:	10.1007/978-1-4939-6747-6_2
Abstrakt:	Enhanced Filter Aided Sample Preparation (eFASP) incorporates plastics passivation and digestion-enhancing surfactants into the traditional FASP workflow to reduce sample loss and increase hydrophobic protein representation in qualitative and quantitative proteomics experiments. Resulting protein digests are free of contaminants and can be analyzed directly by LC-MS.
Databáze:	MEDLINE
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