Improved tympanic membrane regeneration after myringoplastic surgery using an artificial biograft.

Autor: Immich APS; Departamento de Química Fundamental, Instituto de Química, Universidade de São Paulo, São Paulo, Brazil., Pennacchi PC; Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, São Paulo, Brazil., Naves AF; Departamento de Química Fundamental, Instituto de Química, Universidade de São Paulo, São Paulo, Brazil., Felisbino SL; Departamento de Morfologia, Instituto de Biociências de Botucatu, Universidade Estadual Paulista 'Júlio de Mesquita Filho', Botucatu, Brazil., Boemo RL; Centro de Otorrinolaringologia e Fonoaudiologia de São Paulo, Complexo Hospitalar Edmundo Vasconcelos, São Paulo, Brazil., Maria-Engler SS; Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, São Paulo, Brazil., Catalani LH; Departamento de Química Fundamental, Instituto de Química, Universidade de São Paulo, São Paulo, Brazil. Electronic address: catalani@usp.br.
Jazyk: angličtina
Zdroj: Materials science & engineering. C, Materials for biological applications [Mater Sci Eng C Mater Biol Appl] 2017 Apr 01; Vol. 73, pp. 48-58. Date of Electronic Publication: 2016 Dec 06.
DOI: 10.1016/j.msec.2016.12.007
Abstrakt: Tympanic membrane perforations are due to common otologic problems. The current treatments to heal tympanic membrane perforation, such as myringoplasty, have some disadvantages, including the need for autologous grafting, which is rapidly absorbed by the organism before perforation recovery is complete. To improve the structural and functional tympanic membrane healing after surgery, we propose a new branch of artificial grafts. In this study, we report the development of artificial grafts using electrospun bioabsorbable polymers. Polymers such as poly (l-lactic acid) and poly (lactic-co-glycolic acid) acted as the scaffold for cell growth in a co-culture of fibroblasts and keratinocytes. This co-culture promoted the growth of an epithelial-equivalent tissue over the electrospun scaffold, which was used as an alternative graft in myringoplasty. The in vivo study was performed in Sprague Dawley rats. Ear endoscopy was performed 30days after surgery and showed that tympanic membrane perforations treated with artificial grafts healed naturally, completely and with the possibility of maintaining their actual functionality. In conclusion, our study described a new artificial graft created specifically to fulfill the requirements of perforated tympanic membrane healing processes, which are compatibility, proper durability and less intense side effects following myringoplasty.
(Copyright © 2016 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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