| Autor: |
Rodrigues MFC; a Department of Physiological Sciences, Federal University of São Carlos, UFSCar, São Carlos, São Paulo 13565-905, Brazil., Ferreira FC; a Department of Physiological Sciences, Federal University of São Carlos, UFSCar, São Carlos, São Paulo 13565-905, Brazil., Silva-Magosso NS; a Department of Physiological Sciences, Federal University of São Carlos, UFSCar, São Carlos, São Paulo 13565-905, Brazil., Barbosa MR; c Department of Health Education, Federal University of Sergipe, Lagarto, Sergipe 49400-000, Brazil., Souza MVC; a Department of Physiological Sciences, Federal University of São Carlos, UFSCar, São Carlos, São Paulo 13565-905, Brazil., Domingos MM; a Department of Physiological Sciences, Federal University of São Carlos, UFSCar, São Carlos, São Paulo 13565-905, Brazil., Canevazzi GHR; a Department of Physiological Sciences, Federal University of São Carlos, UFSCar, São Carlos, São Paulo 13565-905, Brazil., Stotzer US; a Department of Physiological Sciences, Federal University of São Carlos, UFSCar, São Carlos, São Paulo 13565-905, Brazil., Peviani SM; a Department of Physiological Sciences, Federal University of São Carlos, UFSCar, São Carlos, São Paulo 13565-905, Brazil., de Lira FS; b Exercise and Immunometabolism Research Group, Department of Physical Education, University State Estadual Paulista, UNESP, Presidente Prudente, São Paulo 19060-900, Brazil., Selistre de Araújo HS; a Department of Physiological Sciences, Federal University of São Carlos, UFSCar, São Carlos, São Paulo 13565-905, Brazil., Perez SEA; a Department of Physiological Sciences, Federal University of São Carlos, UFSCar, São Carlos, São Paulo 13565-905, Brazil. |
| Abstrakt: |
Estrogen deficiency is directly related to central obesity and low-grade inflammation. Hormonal replacement and exercise training are both able to decrease fat accumulation and inflammation in postmenopausal women. However, the efficiency of resistance training (RT) and estrogen replacement (ER) in minimizing adiposity and inflammation in the visceral adipose tissue (VAT) of ovariectomized (OVX) rats has not yet been elucidated. In this study, Sprague-Dawley rats were divided into the following 6 groups: sham-operated sedentary (Sham-Sed), OVX-Sed, Sham-RT, OVX-RT, OVX-Sed-ER, and OVX-RT-ER groups. ER was performed by implanting silastic capsules containing 17β-estradiol. For RT, the animals were required to climb a 1.1-m vertical ladder with conical flasks containing weights attached to their tails for 12 weeks. Histological analyses were used to evaluate morphological changes. Gene expression levels were determined by quantitative real-time reverse transcriptase polymerase chain reaction, and protein concentrations were determined using Multiplex/Luminex assays. Ovariectomy increased the body mass (BM), adipocyte area, and inflammation in the VAT, the latter of which was indicated by reduced interleukin-10 (48%) and increased tumor necrosis factor (TNF)-α concentration (∼3%). RT efficiently decreased BM, adipocyte area, and inflammation in the OVX groups. The combination of RT and ER decreased BM (19%) and the TNF-α concentration (18%) and increased the gene and protein expression levels of adiponectin (173% and 18%). These results indicate that RT and the combination of RT and ER are efficient strategies for reducing the BM and improving the inflammatory status of OVX rats. |
