Elastic modulus and hydraulic permeability of MDCK monolayers.

Autor: Schulze KD; Department of Mechanical and Aerospace Engineering, University of Florida, Gainesville, FL, United States., Zehnder SM; Department of Mechanical and Aerospace Engineering, University of Florida, Gainesville, FL, United States., Urueña JM; Department of Mechanical and Aerospace Engineering, University of Florida, Gainesville, FL, United States., Bhattacharjee T; Department of Mechanical and Aerospace Engineering, University of Florida, Gainesville, FL, United States., Sawyer WG; Department of Mechanical and Aerospace Engineering, University of Florida, Gainesville, FL, United States; Department of Material Science and Engineering, University of Florida, Gainesville, FL, United States., Angelini TE; Department of Mechanical and Aerospace Engineering, University of Florida, Gainesville, FL, United States; J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL, United States; Institute for Cell Engineering and Regenerative Medicine, University of Florida, Gainesville, FL, United States. Electronic address: t.e.angelini@ufl.edu.
Jazyk: angličtina
Zdroj: Journal of biomechanics [J Biomech] 2017 Feb 28; Vol. 53, pp. 210-213. Date of Electronic Publication: 2017 Jan 20.
DOI: 10.1016/j.jbiomech.2017.01.016
Abstrakt: The critical role of cell mechanics in tissue health has led to the development of many in vitro methods that measure the elasticity of the cytoskeleton and whole cells, yet the connection between these local cell properties and bulk measurements of tissue mechanics remains unclear. To help bridge this gap, we have developed a monolayer indentation technique for measuring multi-cellular mechanics in vitro. Here, we measure the elasticity of cell monolayers and uncover the role of fluid permeability in these multi-cellular systems, finding that the resistance of fluid transport through cells controls their force-response at long times.
(Copyright © 2017 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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