Experimental murine model of renal cancer.

Autor:	Padilla-Fernández B; Servicio de Urología, Hospital Universitario de Canarias, Tenerife, España., García-Cenador MB; Departamento de Cirugía, Universidad de Salamanca, Salamanca, España., Rodríguez-Marcos P; Departamento de Cirugía, Universidad de Salamanca, Salamanca, España., López-Marcos JF; Departamento de Cirugía, Universidad de Salamanca, Salamanca, España., Antúnez-Plaza P; Servicio de Anatomía Patológica, Hospital Universitario de Salamanca, Salamanca, España., Silva-Abuín JM; Servicio de Urología, Hospital San Pedro, Logroño, España., López-Montañés D; Departamento de Cirugía, Universidad de Salamanca, Salamanca, España., García-Criado FJ; Departamento de Cirugía, Universidad de Salamanca, Salamanca, España., Lorenzo-Gómez MF; Departamento de Cirugía, Universidad de Salamanca, Salamanca, España. Electronic address: mflorenzogo@yahoo.es.
Jazyk:	English; Spanish; Castilian
Zdroj:	Actas urologicas espanolas [Actas Urol Esp] 2017 Sep; Vol. 41 (7), pp. 445-450. Date of Electronic Publication: 2017 Feb 02.
DOI:	10.1016/j.acuro.2016.11.005
Abstrakt:	Introduction: The objective of this study was to determine the reproducibility in a murine model of renal tumours of various histological strains that could be useful for investigating the response to target drugs. Material and Methods: Development and analysis of the "in vivo" model: tumour xenograft of renal cell carcinomas with Balb/c nude athymic mice. Nontumourous human renal tissue was implanted in the interscapular region of 5 mice, chromophobe renal cell carcinoma was implanted in 5 mice (which, after checking its growth, was prepared for implantation in another 10 mice) and Fuhrman grade 2 clear cell renal cell carcinoma (CCRCC) was implanted in 5 mice (which was also subsequently implanted in 10 mice). We monitored the tumour size, onset of metastases and increase in size and number of tumours. When the size had reached a point greater than or equal to locally advanced or metastatic carcinoma, the animals were euthanised for a pathological and immunohistochemical study and a second phase of implantation. Results: The subcutaneous xenograft of the healthy tissue did not grow. The animals were euthanised at 6 months and no renal tissue was found. The chromophobe renal cell carcinoma cells grew in the initial phase (100%); however, in the second phase, we observed a chronic lymphomonocyte inflammatory reaction and a foreign body reaction. The CCRCC grew at 5-8 months both in the first and second phase (100%), maintaining the tumour type and grade. Conclusions: The model with athymic Balb/c nude mice is useful for reproducing CCRCC, with the same histological characteristics and aggressiveness as native human tumours, promoting the development of the second experimental phase. (Copyright © 2016 AEU. Publicado por Elsevier España, S.L.U. All rights reserved.)
Databáze:	MEDLINE
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