A two-column flash chromatography approach to pyoverdin production from Pseudomonas putida GB1.

Autor: Duckworth OW; Department of Crop and Soil Sciences, North Carolina State University, Raleigh, NC 27695, USA. Electronic address: owen_duckworth@ncsu.edu., Markarian DS; Department of Crop and Soil Sciences, North Carolina State University, Raleigh, NC 27695, USA., Parker DL; Scripps Institution of Oceanography, University of California, La Jolla, San Diego, CA 92093, USA., Harrington JM; RTI International, 3040 East Cornwallis Road, Durham, NC 27709, USA.
Jazyk: angličtina
Zdroj: Journal of microbiological methods [J Microbiol Methods] 2017 Apr; Vol. 135, pp. 11-13. Date of Electronic Publication: 2017 Feb 01.
DOI: 10.1016/j.mimet.2017.01.019
Abstrakt: Our knowledge of the biological and environmental reactivity of siderophores is limited by the difficulty and cost of obtaining reasonable quantities by purification or synthesis. In this note, we describe a modified procedure for the low-cost, mg-scale purification of pyoverdin-type siderophores using a dual-flash chromatography (reverse-phase absorption and size exclusion) approach.
(Copyright © 2017 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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