Citotoxicity evaluation of three dental adhesives on vero cells in vitro .

Autor:	Catunda RQ; MSc student, Department of Clinical and Preventive Dentistry, Federal University of Pernambuco, Recife, Pernambuco, Brazil., Vieira JR; Professor, Department of Histology and Embryology, Federal University of Pernambuco, Recife, Pernambuco, Brazil., de Oliveira EB; Professor, Department of Histology and Embryology, Federal University of Pernambuco, Recife, Pernambuco, Brazil., da Silva EC; Undergraduate, Department of Histology and Embryology, Federal University of Pernambuco, Recife, Pernambuco, Brazil., Brasil VL; PhD student, Department of Clinical and Preventive Dentistry, Federal University of Pernambuco, Recife, Pernambuco, Brazil., Perez DC; Professor, Department of Clinical and Preventive Dentistry, Federal University of Pernambuco, Recife, Pernambuco, Brazil.
Jazyk:	angličtina
Zdroj:	Journal of clinical and experimental dentistry [J Clin Exp Dent] 2017 Jan 01; Vol. 9 (1), pp. e61-e66. Date of Electronic Publication: 2017 Jan 01 (Print Publication: 2017).
DOI:	10.4317/jced.53039
Abstrakt:	Background: To evaluate, in vitro , the potential cytotoxicity of three different dental adhesives systems (Adper Single Bond 2 -SB, Silorane System Adhesive Bond -SSAB and Single Bond Universal -SBU) on cultivated Vero cells after different contact times. Material and Methods: The cells were cultured in a concentration of 2 x 105 cells/mL for 24h and grown to sub-confluent monolayers. VERO cells were exposed to 25µl of conditioned extracts obtained from 24h, 48h and 72h immersion of adhesive samples in culture medium (DMEM), immediately after polymerization. Fresh DMEM was used as negative control. Cell metabolism was evaluated by the MTT assay (3-(4,5-dimethylthiazol-2-yl)-2, 5diphenyl-tetrazolium bromide). The data were analyzed statistically by ANOVA, considering a significance of 5%. Results: The values of cell viability ranged from 94.2% at 72h (SBU) to 109.6% at 48h (SB). The mean percentage of viability after exposure to the extracts of SB, SSAB and SBU were 103.2%, 100.63% and 97.43%, respectively. There was no statistically significant difference ( p = 0.342) between the experimental and negative control groups. Conclusions: At all exposure times, all adhesives tested in this study presented no cytotoxicity to Vero cells in vitro . Key words: Biocompatibility, cytotoxicity, dental adhesives, Vero cells. Competing Interests: The authors have declared that no conflict of interest exist.
Databáze:	MEDLINE
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