Comparison between Radionuclide Ventriculography and Echocardiography for Quantification of Left Ventricular Systolic Function in Rats Exposed to Doxorubicin.

Autor:	Oliveira LF; Centro de Cardiologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Brazil., O'Connell JL; Universidade Federal de Uberlândia, MG, Brazil., Carvalho EE; Centro de Cardiologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Brazil., Pulici ÉC; Universidade Federal de Uberlândia, MG, Brazil., Romano MM; Centro de Cardiologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Brazil., Maciel BC; Centro de Cardiologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Brazil., Simões MV; Centro de Cardiologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Brazil.
Jazyk:	Portuguese; English
Zdroj:	Arquivos brasileiros de cardiologia [Arq Bras Cardiol] 2017 Jan; Vol. 108 (1), pp. 12-20.
DOI:	10.5935/abc.20160194
Abstrakt:	Background: Radionuclide ventriculography (RV) is a validated method to evaluate the left ventricular systolic function (LVSF) in small rodents. However, no prior study has compared the results of RV with those obtained by other imaging methods in this context. Objectives: To compare the results of LVSF obtained by RV and echocardiography (ECHO) in an experimental model of cardiotoxicity due to doxorubicin (DXR) in rats. Methods: Adult male Wistar rats serving as controls (n = 7) or receiving DXR (n = 22) in accumulated doses of 8, 12, and 16 mg/kg were evaluated with ECHO performed with a Sonos 5500 Philips equipment (12-MHz transducer) and RV obtained with an Orbiter-Siemens gamma camera using a pinhole collimator with a 4-mm aperture. Histopathological quantification of myocardial fibrosis was performed after euthanasia. Results: The control animals showed comparable results in the LVSF analysis obtained with ECHO and RV (83.5 ± 5% and 82.8 ± 2.8%, respectively, p > 0.05). The animals that received DXR presented lower LVSF values when compared with controls (p < 0.05); however, the LVSF values obtained by RV (60.6 ± 12.5%) were lower than those obtained by ECHO (71.8 ± 10.1%, p = 0.0004) in this group. An analysis of the correlation between the LVSF and myocardial fibrosis showed a moderate correlation when the LVSF was assessed by ECHO (r = -0.69, p = 0.0002) and a stronger correlation when it was assessed by RV (r = -0.79, p < 0.0001). On multiple regression analysis, only RV correlated independently with myocardial fibrosis. Conclusion: RV is an alternative method to assess the left ventricular function in small rodents in vivo. When compared with ECHO, RV showed a better correlation with the degree of myocardial injury in a model of DXR-induced cardiotoxicity. Competing Interests: Potential Conflict of Interest No potential conflict of interest relevant to this article was reported.
Databáze:	MEDLINE
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