Effects on breathing of agonists to μ-opioid or GABA A receptors dialyzed into the ventral respiratory column of awake and sleeping goats.

Autor: Langer TM 3rd; Department of Physiology, Medical College of Wisconsin, Milwaukee, WI 53226, United States., Neumueller SE; Department of Physiology, Medical College of Wisconsin, Milwaukee, WI 53226, United States., Crumley E; Department of Physiology, Medical College of Wisconsin, Milwaukee, WI 53226, United States., Burgraff NJ; Department of Physiology, Medical College of Wisconsin, Milwaukee, WI 53226, United States., Talwar S; Department of Physiology, Medical College of Wisconsin, Milwaukee, WI 53226, United States., Hodges MR; Department of Physiology, Medical College of Wisconsin, Milwaukee, WI 53226, United States; Neuroscience Research Center, Medical College of Wisconsin, Milwaukee, WI 53226, United States., Pan L; Department of Physiology, Medical College of Wisconsin, Milwaukee, WI 53226, United States; Department of Physical Therapy, Marquette University, Milwaukee, WI 53226, United States., Forster HV; Department of Physiology, Medical College of Wisconsin, Milwaukee, WI 53226, United States; Neuroscience Research Center, Medical College of Wisconsin, Milwaukee, WI 53226, United States; Zablocki Veterans Affairs Medical Center, Milwaukee, WI 53226, United States. Electronic address: bforster@mcw.edu.
Jazyk: angličtina
Zdroj: Respiratory physiology & neurobiology [Respir Physiol Neurobiol] 2017 May; Vol. 239, pp. 10-25. Date of Electronic Publication: 2017 Jan 27.
DOI: 10.1016/j.resp.2017.01.007
Abstrakt: Pulmonary ventilation (V̇ I ) in awake and sleeping goats does not change when antagonists to several excitatory G protein-coupled receptors are dialyzed unilaterally into the ventral respiratory column (VRC). Concomitant changes in excitatory neuromodulators in the effluent mock cerebral spinal fluid (mCSF) suggest neuromodulatory compensation. Herein, we studied neuromodulatory compensation during dialysis of agonists to inhibitory G protein-coupled or ionotropic receptors into the VRC. Microtubules were implanted into the VRC of goats for dialysis of mCSF mixed with agonists to either μ-opioid (DAMGO) or GABA A (muscimol) receptors. We found: (1) V̇ I decreased during unilateral but increased during bilateral dialysis of DAMGO, (2) dialyses of DAMGO destabilized breathing, (3) unilateral dialysis of muscimol increased V̇ I, and (4) dialysis of DAMGO decreased GABA in the effluent mCSF. We conclude: (1) neuromodulatory compensation can occur during altered inhibitory neuromodulator receptor activity, and (2) the mechanism of compensation differs between G protein-coupled excitatory and inhibitory receptors and between G protein-coupled and inotropic inhibitory receptors.
(Copyright © 2017 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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