Evidence that d-cysteine protects mice from gastric damage via hydrogen sulfide produced by d-amino acid oxidase.

Autor: Souza LK; Northeast Biotechnology Network (RENORBIO), Postgraduate Program in Biotechnology, Federal University of Piauí, Parnaíba, Piauí, Brazil; Biotechnology and Biodiversity Center Research, BIOTEC, Postgraduate Program in Biotechnology, Federal University of Piauí, Parnaíba, Piauí, Brazil., Araújo TS; Northeast Biotechnology Network (RENORBIO), Postgraduate Program in Biotechnology, Federal University of Piauí, Parnaíba, Piauí, Brazil; Biotechnology and Biodiversity Center Research, BIOTEC, Postgraduate Program in Biotechnology, Federal University of Piauí, Parnaíba, Piauí, Brazil., Sousa NA; Northeast Biotechnology Network (RENORBIO), Postgraduate Program in Biotechnology, Federal University of Piauí, Parnaíba, Piauí, Brazil., Sousa FB; Biotechnology and Biodiversity Center Research, BIOTEC, Postgraduate Program in Biotechnology, Federal University of Piauí, Parnaíba, Piauí, Brazil., Nogueira KM; Biotechnology and Biodiversity Center Research, BIOTEC, Postgraduate Program in Biotechnology, Federal University of Piauí, Parnaíba, Piauí, Brazil., Nicolau LA; Department of Physiology and Pharmacology, Laboratory of Pharmacology of Inflammation and Cancer (LAFICA), Federal University of Ceará, Fortaleza, CE, Brazil., Medeiros JV; Northeast Biotechnology Network (RENORBIO), Postgraduate Program in Biotechnology, Federal University of Piauí, Parnaíba, Piauí, Brazil; Biotechnology and Biodiversity Center Research, BIOTEC, Postgraduate Program in Biotechnology, Federal University of Piauí, Parnaíba, Piauí, Brazil. Electronic address: jandvenes@ufpi.edu.br.
Jazyk: angličtina
Zdroj: Nitric oxide : biology and chemistry [Nitric Oxide] 2017 Apr 01; Vol. 64, pp. 1-6. Date of Electronic Publication: 2017 Jan 27.
DOI: 10.1016/j.niox.2017.01.010
Abstrakt: Hydrogen sulfide (H 2 S) is a signaling molecule in the gastrointestinal tract. H 2 S production can derive from d-cysteine via various pathways, thus pointing to a new therapeutic approach: delivery of H 2 S to specific tissues. This study was designed to evaluate the concentration and effects of H 2 S (generated by d-amino acid oxidase [DAO] from d-cysteine) in the gastric mucosa and the protective effects against ethanol-induced lesions in mice. Mice were treated with l-cysteine or d-cysteine (100 mg/kg per os). Other groups received oral l-propargylglycine (cystathionine γ-lyase inhibitor, 100 mg/kg) or indole-2-carboxylate (DAO inhibitor), and 30 min later, received d- or l-cysteine. After 30 min, 50% ethanol (2.5 mL/kg, per os) was administered. After 1 h, the mice were euthanized and their stomachs excised and analyzed. Pretreatment with either l-cysteine or d-cysteine significantly reduced ethanol-induced lesions. Pretreatment of d-cysteine- or l-cysteine-treated groups with indole-2-carboxylate reversed the gastroprotective effects of d-cysteine but not l-cysteine. Histological analysis revealed that pretreatment with d-cysteine decreased hemorrhagic damage, edema, and the loss of the epithelium, whereas the administration of indole-2-carboxylate reversed these effects. d-Cysteine also reduced malondialdehyde levels but maintained the levels of reduced glutathione. Furthermore, pretreatment with d-cysteine increased the synthesis of H 2 S. Thus, an H 2 S-generating pathway (involving d-cysteine and DAO) is present in the gastric mucosa and protects this tissue from ethanol-induced damage by decreasing direct oxidative damage.
(Copyright © 2017 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: