Oligomerization affects the kinetics and thermodynamics of the interaction of a Bowman-Birk inhibitor with proteases.

Autor: Brand GD; Laboratório de Espectrometria de Massa, EMBRAPA - Recursos Genéticos e Biotecnologia, Brasília, DF, Brazil; Laboratório de Síntese e Análise de Biomoléculas, Instituto de Química, Universidade de Brasília, Brasília, DF, Brazil. Electronic address: gdbrand@unb.br., Pires DA; Laboratório de Espectrometria de Massa, EMBRAPA - Recursos Genéticos e Biotecnologia, Brasília, DF, Brazil; Instituto Federal de Educação, Ciência e Tecnologia de Goiás, Luziânia, GO, Brazil., Furtado JR Jr; Departamento de Polícia Federal, Unidade Técnico-Científica, Londrina, PR, Brazil., Cooper A; School of Chemistry, University of Glasgow, Glasgow G12 8QQ, Scotland, UK., Freitas SM; Laboratório de Biofísica, Instituto de Biologia, Universidade de Brasília, Brasília, DF, Brazil., Bloch C Jr; Laboratório de Espectrometria de Massa, EMBRAPA - Recursos Genéticos e Biotecnologia, Brasília, DF, Brazil.
Jazyk: angličtina
Zdroj: Archives of biochemistry and biophysics [Arch Biochem Biophys] 2017 Mar 15; Vol. 618, pp. 9-14. Date of Electronic Publication: 2017 Jan 26.
DOI: 10.1016/j.abb.2017.01.009
Abstrakt: The black-eyed pea trypsin/chymotrypsin inhibitor (BTCI) forms concentration dependent homomultimers, as previously demonstrated by Light scattering and Atomic Force Microscopy. Considering that these self-aggregates might influence their binding to cognate enzymes, we investigated the interaction of BTCI at picomolar concentrations using surface immobilized Chymotrypsin (α-CT) and Trypsin (T) by Surface Plasmon Resonance. Our results indicate that BTCI has subnanomolar affinity to both immobilized enzymes, which is approximately two orders of magnitude higher than previously reported. Moreover, we probed the influence of temperature on protein binding equilibria in order to investigate their interaction energetics. While the BTCI/T interaction concurs with the canonical entropy-driven mechanism described for BBI interactions with serine proteinases, the BTCI/α-CT interaction does not. Our measurements indicate that bimolecular BTCI/α-CT complexes form with a negative enthalpy change and a moderate entropic increase. Direct calorimetric evaluation is in accord with the van't Hoff approximation obtained by SPR. We demonstrate that as protein concentrations increase to the micromolar range, secondary endothermic events become prevalent and affect both the kinetics and thermodynamics of protein associations. Our study reinforces that BBI interactions with serine proteinases should be studied in dilute solutions to abridge often neglected secondary interactions.
(Copyright © 2017 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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