Repeated remote ischemic preconditioning and isoflurane anesthesia in an experimental model of renal ischemia-reperfusion injury.

Autor: Menting TP; Department of Surgery, Radboud University Medical Center, Geert Grooteplein-zuid 10, 6525 GA, Nijmegen, The Netherlands. Theo.menting@radboudumc.nl., Ergun M; Department of Surgery, Radboud University Medical Center, Geert Grooteplein-zuid 10, 6525 GA, Nijmegen, The Netherlands., Bruintjes MH; Department of Surgery, Radboud University Medical Center, Geert Grooteplein-zuid 10, 6525 GA, Nijmegen, The Netherlands., Wever KE; Systematic Review Centre for Laboratory animal Experimentation, SYRCLE, Radboud University Medical Center, Geert Grooteplein-zuid 10, 6525 GA, Nijmegen, The Netherlands., Lomme RM; Department of Surgery, Radboud University Medical Center, Geert Grooteplein-zuid 10, 6525 GA, Nijmegen, The Netherlands., van Goor H; Department of Surgery, Radboud University Medical Center, Geert Grooteplein-zuid 10, 6525 GA, Nijmegen, The Netherlands., Warlé MC; Department of Surgery, Radboud University Medical Center, Geert Grooteplein-zuid 10, 6525 GA, Nijmegen, The Netherlands.
Jazyk: angličtina
Zdroj: BMC anesthesiology [BMC Anesthesiol] 2017 Jan 28; Vol. 17 (1), pp. 14. Date of Electronic Publication: 2017 Jan 28.
DOI: 10.1186/s12871-017-0310-x
Abstrakt: Background: In animal studies, remote ischemic preconditioning (RIPC) and anesthetic preconditioning are successful in reducing renal ischemia reperfusion injury (IRI), however the protective effect of RIPC may be improved by repeating the RIPC stimulus.
Methods: Sprague-Dawley rats underwent unilateral nephrectomy followed by 30 min of renal pedicle clamping. Animals were allocated into six groups: sham, control (IRI), RepISO (daily isoflurane anesthesia), RIPC (single dose isoflurane anesthesia and single dose RIPC), RepISO + RIPC (7-day isoflurane anesthesia and single dose RIPC) and RepISO + RepRIPC (7-day isoflurane anesthesia with 7-day RIPC). RIPC was applied by 3×5 min of cuff inflation on both thighs. Serum creatinine and urea levels were measured and histology was obtained at day two.
Results: RepISO diminished renal IRI, as reflected by a significant reduction in serum creatinine levels as compared to the control group, 170 ± 74 resp. 107 ± 29 μmol/L. The other preconditioning protocols showed similar reduction in serum creatinine levels as compared to the control group. No significant differences were observed between the different preconditioning protocols. For urea levels, only RepISO + RIPC resulted in significantly lower levels as compared to the control group, 14 ± 4 resp. 22 ± 7 mmol/L (p = 0.010). In the preconditioning groups only RepISO showed less histological damage as compared to controls 1.73 ± 1.19 resp. 2.91 ± 1.22 (p = 0.032).
Conclusions: In this study no additional protective effect of repeated ischemic preconditioning was observed as compared to single dose RIPC. Repeated administration of isoflurane provided stronger protection against renal IRI as compared to single dose isoflurane.
Databáze: MEDLINE
Externí odkaz: