Mammary Gland Pathology Subsequent to Acute Infection with Strong versus Weak Biofilm Forming Staphylococcus aureus Bovine Mastitis Isolates: A Pilot Study Using Non-Invasive Mouse Mastitis Model.

Autor: Gogoi-Tiwari J; School of Biomedical Sciences, Curtin Health Innovation Research Institute, Curtin University, Bentley, Perth, Western Australia, Australia.; College of Veterinary Sciences and Animal Husbandry, Central Agricultural University, Selesih, Aizawl, Mizoram, India., Williams V; School of Biomedical Sciences, Curtin Health Innovation Research Institute, Curtin University, Bentley, Perth, Western Australia, Australia., Waryah CB; School of Biomedical Sciences, Curtin Health Innovation Research Institute, Curtin University, Bentley, Perth, Western Australia, Australia.; Department of Medicine and Cell Biology, Albert Einstein College of Medicine, Bronx, New York, United States of America., Costantino P; School of Biomedical Sciences, Curtin Health Innovation Research Institute, Curtin University, Bentley, Perth, Western Australia, Australia., Al-Salami H; School of Pharmacy, Curtin Health Innovation Research Institute, Curtin University, Bentley, Perth, Western Australia, Australia., Mathavan S; School of Pharmacy, Curtin Health Innovation Research Institute, Curtin University, Bentley, Perth, Western Australia, Australia., Wells K; School of Biomedical Sciences, Curtin Health Innovation Research Institute, Curtin University, Bentley, Perth, Western Australia, Australia., Tiwari HK; School of Veterinary and Life Sciences, Murdoch University, Perth, Western Australia, Australia., Hegde N; Ella Foundation, Genome Valley, Hyderabad, India., Isloor S; Veterinary College, Karnataka Veterinary, Animal and Fisheries Sciences University, Bangalore, India., Al-Sallami H; School of Pharmacy, University of Otago, Dunedin, New Zealand., Mukkur T; School of Biomedical Sciences, Curtin Health Innovation Research Institute, Curtin University, Bentley, Perth, Western Australia, Australia.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2017 Jan 27; Vol. 12 (1), pp. e0170668. Date of Electronic Publication: 2017 Jan 27 (Print Publication: 2017).
DOI: 10.1371/journal.pone.0170668
Abstrakt: Background: Biofilm formation by Staphylococcus aureus is an important virulence attribute because of its potential to induce persistent antibiotic resistance, retard phagocytosis and either attenuate or promote inflammation, depending upon the disease syndrome, in vivo. This study was undertaken to evaluate the potential significance of strength of biofilm formation by clinical bovine mastitis-associated S. aureus in mammary tissue damage by using a mouse mastitis model.
Methods: Two S. aureus strains of the same capsular phenotype with different biofilm forming strengths were used to non-invasively infect mammary glands of lactating mice. Biofilm forming potential of these strains were determined by tissue culture plate method, ica typing and virulence gene profile per detection by PCR. Delivery of the infectious dose of S. aureus was directly through the teat lactiferous duct without invasive scraping of the teat surface. Both bacteriological and histological methods were used for analysis of mammary gland pathology of mice post-infection.
Results: Histopathological analysis of the infected mammary glands revealed that mice inoculated with the strong biofilm forming S. aureus strain produced marked acute mastitic lesions, showing profuse infiltration predominantly with neutrophils, with evidence of necrosis in the affected mammary glands. In contrast, the damage was significantly less severe in mammary glands of mice infected with the weak biofilm-forming S. aureus strain. Although both IL-1β and TNF-α inflammatory biomarkers were produced in infected mice, level of TNF-α produced was significantly higher (p<0.05) in mice inoculated with strong biofilm forming S. aureus than the weak biofilm forming strain.
Conclusion: This finding suggests an important role of TNF-α in mammary gland pathology post-infection with strong biofilm-forming S. aureus in the acute mouse mastitis model, and offers an opportunity for the development of novel strategies for reduction of mammary tissue damage, with or without use of antimicrobials and/or anti-inflammatory compounds for the treatment of bovine mastitis.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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