Data supporting the effects of lysozyme on mRNA and protein expression in a colonic epithelial scratch wound model.

Autor: Abey SK; Digestive Disorders Unit, Division of Intramural Research, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, USA., Yuana Y; Image Sciences Institute, Division of Imaging, University Medical Centre Utrecht, Netherlands., Joseph PV; Digestive Disorders Unit, Division of Intramural Research, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, USA., Kenea ND; Digestive Disorders Unit, Division of Intramural Research, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, USA., Fourie NH; Digestive Disorders Unit, Division of Intramural Research, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, USA., Sherwin LB; Digestive Disorders Unit, Division of Intramural Research, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, USA., Gonye GE; NanoString Technologies, Seattle, WA, USA., Smyser PA; The Pennsylvania State University, College of Medicine, Hershey, PA, USA., Stempinski ES; Electron Microscopy Core Facility, National Heart, Lung, and Blood Institute, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, USA., Boulineaux CM; Digestive Disorders Unit, Division of Intramural Research, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, USA., Weaver KR; Digestive Disorders Unit, Division of Intramural Research, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, USA., Bleck CK; Electron Microscopy Core Facility, National Heart, Lung, and Blood Institute, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, USA., Henderson WA; Digestive Disorders Unit, Division of Intramural Research, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, USA.
Jazyk: angličtina
Zdroj: Data in brief [Data Brief] 2016 Dec 29; Vol. 11, pp. 15-18. Date of Electronic Publication: 2016 Dec 29 (Print Publication: 2017).
DOI: 10.1016/j.dib.2016.12.043
Abstrakt: Colonic epithelial health is implicated in a host of gastrointestinal (GI) diseases and disorders. Lysozyme is suspected to play a role in the ability of the epithelium to recover from injury (Abey et al., in press; Gallo, 2012; Rubio, 2014) [1], [2], [3]. Disrupted repair mechanisms may lead to delayed or ineffective recovery and disruptions to epithelial biology resulting in GI symptoms and altered barrier function (Peterson and Artis, 2014) [4]. The effect of lysozyme on the transcriptomic and proteomic profile of healthy colonic epithelial cells was investigated. Epithelial cells in culture were scratch wounded and treated with lysozyme. mRNA and protein profiles were simultaneously quantified in the same sample using a digital counting technology. Gene and protein expressions altered by the presence or absence of lysozyme are described in this article. Extensive statistical and bioinformatic analysis, and interpretation of the results can be found in "Lysozyme association with circulating RNA, extracellular vesicles, and chronic stress" (Abey et al., in press) [1].
Databáze: MEDLINE