Myogenic potential of human alveolar mucosa derived cells.

Autor:	Zorin VL; a FSBI 'Central Clinical Hospital with Outpatient Health Center' , Moscow , Russia.; b Department of Regenerative Medicine at the Human Stem Cells Institute , Moscow , Russia., Pulin AA; a FSBI 'Central Clinical Hospital with Outpatient Health Center' , Moscow , Russia., Eremin II; a FSBI 'Central Clinical Hospital with Outpatient Health Center' , Moscow , Russia., Korsakov IN; a FSBI 'Central Clinical Hospital with Outpatient Health Center' , Moscow , Russia., Zorina AI; b Department of Regenerative Medicine at the Human Stem Cells Institute , Moscow , Russia., Khromova NV; c Blokhin Russian Cancer Research Center , Moscow , Russia., Sokova OI; c Blokhin Russian Cancer Research Center , Moscow , Russia., Kotenko KV; d FSBI 'Central State Medical Academy' of Business Administration for the President of the Russian Federation , Moscow , Russia., Kopnin PB; c Blokhin Russian Cancer Research Center , Moscow , Russia.
Jazyk:	angličtina
Zdroj:	Cell cycle (Georgetown, Tex.) [Cell Cycle] 2017 Mar 19; Vol. 16 (6), pp. 545-555. Date of Electronic Publication: 2017 Jan 24.
DOI:	10.1080/15384101.2017.1284714
Abstrakt:	Difficulties related to the obtainment of stem/progenitor cells from skeletal muscle tissue make the search for new sources of myogenic cells highly relevant. Alveolar mucosa might be considered as a perspective candidate due to availability and high proliferative capacity of its cells. Human alveolar mucosa cells (AMC) were obtained from gingival biopsy samples collected from 10 healthy donors and cultured up to 10 passages. AMC matched the generally accepted multipotent mesenchymal stromal cells criteria and possess population doubling time, caryotype and immunophenotype stability during long-term cultivation. The single myogenic induction of primary cell cultures resulted in differentiation of AMC into multinucleated myotubes. The myogenic differentiation was associated with expression of skeletal muscle markers: skeletal myosin, skeletal actin, myogenin and MyoD1. Efficiency of myogenic differentiation in AMC cultures was similar to that in skeletal muscle cells. Furthermore, some of differentiated myotubes exhibited contractions in vitro. Our data confirms the sufficiently high myogenic potential and proliferative capacity of AMC and their ability to maintain in vitro proliferation-competent myogenic precursor cells regardless of the passage number.
Databáze:	MEDLINE
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