The Zebrafish Anillin-eGFP Reporter Marks Late Dividing Retinal Precursors and Stem Cells Entering Neuronal Lineages.

Autor: Cepero Malo M; Centre for Organismal Studies, Heidelberg University, Heidelberg, Germany., Duchemin AL; Centre for Organismal Studies, Heidelberg University, Heidelberg, Germany., Guglielmi L; Department of Cell and Molecular Biology, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany., Patzel E; Centre for Organismal Studies, Heidelberg University, Heidelberg, Germany., Sel S; The David J Apple Center for Vision Research, Department of Ophthalmology, Heidelberg University Hospital, Heidelberg, Germany., Auffarth GU; The David J Apple Center for Vision Research, Department of Ophthalmology, Heidelberg University Hospital, Heidelberg, Germany., Carl M; Department of Cell and Molecular Biology, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany., Poggi L; Centre for Organismal Studies, Heidelberg University, Heidelberg, Germany.; The David J Apple Center for Vision Research, Department of Ophthalmology, Heidelberg University Hospital, Heidelberg, Germany.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2017 Jan 20; Vol. 12 (1), pp. e0170356. Date of Electronic Publication: 2017 Jan 20 (Print Publication: 2017).
DOI: 10.1371/journal.pone.0170356
Abstrakt: Monitoring cycling behaviours of stem and somatic cells in the living animal is a powerful tool to better understand tissue development and homeostasis. The tg(anillin:anillin-eGFP) transgenic line carries the full-length zebrafish F-actin binding protein Anillin fused to eGFP from a bacterial artificial chromosome (BAC) containing Anillin cis-regulatory sequences. Here we report the suitability of the Anillin-eGFP reporter as a direct indicator of cycling cells in the late embryonic and post-embryonic retina. We show that combining the anillin:anillin-eGFP with other transgenes such as ptf1a:dsRed and atoh7:gap-RFP allows obtaining spatial and temporal resolution of the mitotic potentials of specific retinal cell populations. This is exemplified by the analysis of the origin of the previously reported apically and non-apically dividing late committed precursors of the photoreceptor and horizontal cell layers.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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