| Autor: |
Noman L; a Laboratory of Natural Products and Organic Synthesis, Department of Chemistry, Faculty of Science , University of Mentouri-Constantine , Constantine , Algeria.; d Laboratory of Plant Research, Department of Chemistry, Faculty of Science , Cankiri Karatekin University , Cankiri , Turkey., Oke-Altuntas F; b Department of Biology, Faculty of Science , Gazi University , Ankara , Turkey., Zellagui A; a Laboratory of Natural Products and Organic Synthesis, Department of Chemistry, Faculty of Science , University of Mentouri-Constantine , Constantine , Algeria.; c Laboratory of Biomolecules and Plant Breeding, Life Science and Nature Department, Faculty of Exact Science and Life Science and Nature , University of Larbi Ben Mhidi Oum El Bouaghi , Oum El Bouaghi , Algeria., Sahin Yaglioglu A; d Laboratory of Plant Research, Department of Chemistry, Faculty of Science , Cankiri Karatekin University , Cankiri , Turkey., Demirtas I; d Laboratory of Plant Research, Department of Chemistry, Faculty of Science , Cankiri Karatekin University , Cankiri , Turkey., M Cardoso S; e Department of Chemistry & QOPNA , University of Aveiro , Aveiro , Portugal., Akkal S; f Departement de Chimie, Facultés des Sciences exactes , Université de Constantine, Unité de Recherche Valorisation des ressources naturelles Molécules bioactives et Analyses Physico-Chimiques et Biologiques , Constantine , Algéria., Gherraf N; a Laboratory of Natural Products and Organic Synthesis, Department of Chemistry, Faculty of Science , University of Mentouri-Constantine , Constantine , Algeria.; c Laboratory of Biomolecules and Plant Breeding, Life Science and Nature Department, Faculty of Exact Science and Life Science and Nature , University of Larbi Ben Mhidi Oum El Bouaghi , Oum El Bouaghi , Algeria., Rhouati S; a Laboratory of Natural Products and Organic Synthesis, Department of Chemistry, Faculty of Science , University of Mentouri-Constantine , Constantine , Algeria. |
| Abstrakt: |
A new compound (microphybenzimidazole, 7) along with the six known compounds matairesinol (1), prestegane B (2), umbelliferone (3), daphnoretin (4), microphynolide A (5) and microphynolide B (6) were isolated from Thymelaea microphylla. The structures of the pure compounds were elucidated by spectroscopic and mass-spectrometric analyses, including 1D-, 2D-NMR, and HPLC-TOF/MS. Compounds 2 and 4, as well as three fractions (F6, F6-C5, and F6-W42) obtained from a 50% (v:v) CH 2 Cl 2 :MeOH extract exhibited a selective activity against rat brain glioma cells (C6). Moreover, compound 1 and other fractions obtained from 50% (v:v) CH 2 Cl 2 :MeOH and 70% (v:v) MeOH:H 2 O extracts exhibited dose- and time-dependent effects on human cervical cancer cell (HeLa), as measured by xCELLigence assay. Compound 2 (IC 50 = 14.0 ± 0.2 μg/mL) and fraction F5 (IC 50 = 12.4 ± 0.1 μg/mL) showed higher radical scavenging ability than the synthetic agent butylated hydroxytoluene (BHT, IC 50 = 22.7 ± 0.6 μg/mL). |
