The Goddard and Saturn Genes Are Essential for Drosophila Male Fertility and May Have Arisen De Novo.

Autor:	Gubala AM; Department of Biology, College of the Holy Cross, Worcester, MA., Schmitz JF; Evolutionary Bioinformatics Group, Institute for Evolution and Biodiversity, University of Münster, Münster, Germany., Kearns MJ; Department of Biology, College of the Holy Cross, Worcester, MA., Vinh TT; Department of Biology, College of the Holy Cross, Worcester, MA., Bornberg-Bauer E; Evolutionary Bioinformatics Group, Institute for Evolution and Biodiversity, University of Münster, Münster, Germany., Wolfner MF; Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY., Findlay GD; Department of Biology, College of the Holy Cross, Worcester, MA.; Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY.
Jazyk:	angličtina
Zdroj:	Molecular biology and evolution [Mol Biol Evol] 2017 May 01; Vol. 34 (5), pp. 1066-1082.
DOI:	10.1093/molbev/msx057
Abstrakt:	New genes arise through a variety of mechanisms, including the duplication of existing genes and the de novo birth of genes from noncoding DNA sequences. While there are numerous examples of duplicated genes with important functional roles, the functions of de novo genes remain largely unexplored. Many newly evolved genes are expressed in the male reproductive tract, suggesting that these evolutionary innovations may provide advantages to males experiencing sexual selection. Using testis-specific RNA interference, we screened 11 putative de novo genes in Drosophila melanogaster for effects on male fertility and identified two, goddard and saturn, that are essential for spermatogenesis and sperm function. Goddard knockdown (KD) males fail to produce mature sperm, while saturn KD males produce few sperm, and these function inefficiently once transferred to females. Consistent with a de novo origin, both genes are identifiable only in Drosophila and are predicted to encode proteins with no sequence similarity to any annotated protein. However, since high levels of divergence prevented the unambiguous identification of the noncoding sequences from which each gene arose, we consider goddard and saturn to be putative de novo genes. Within Drosophila, both genes have been lost in certain lineages, but show conserved, male-specific patterns of expression in the species in which they are found. Goddard is consistently found in single-copy and evolves under purifying selection. In contrast, saturn has diversified through gene duplication and positive selection. These data suggest that de novo genes can acquire essential roles in male reproduction. (© The Author 2017. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
Databáze:	MEDLINE
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