| Autor: |
Al-Rasheed NM; a Pharmacology Department, Faculty of Pharmacy , King Saud University , Riyadh , Kingdom of Saudi Arabia., El-Orabi NF; a Pharmacology Department, Faculty of Pharmacy , King Saud University , Riyadh , Kingdom of Saudi Arabia.; b Department of Pharmacology and Toxicology, Faculty of Pharmacy , Suez Canal University , Ismailia , Egypt., Fadda LM; a Pharmacology Department, Faculty of Pharmacy , King Saud University , Riyadh , Kingdom of Saudi Arabia., Ali HM; c Department of Genetics and Cytology , National Research Center , Dokki , Egypt.; d Preparatory Year Deanship , King Saud University , Riyadh , Kingdom of Saudi Arabia., Al-Rasheed NM; a Pharmacology Department, Faculty of Pharmacy , King Saud University , Riyadh , Kingdom of Saudi Arabia., Bassiouni Y; a Pharmacology Department, Faculty of Pharmacy , King Saud University , Riyadh , Kingdom of Saudi Arabia., Aldbass AM; e Biochemistry Department, Faculty of Science , King Saud University , Riyadh , Kingdom of Saudi Arabia. |
| Abstrakt: |
Overexpression of nuclear factor (NF-κB) or activation of Smad3 by transforming growth factor β (TGF-β1) induced by oncogenes results in overexpression of fibrotic processes and hence cell death. The objective of this study is to examine whether Silymarin (Sil) alone or in combination with Vitamin E (Vit E) and/or Curcumin (Cur) plays a modulatory role against the overexpression of NF-κB, and TGF-β that induced in response to carbon tetrachloride (CCl 4 ) administration. The present work revealed that CCl 4 induced elevation of in serum alanine aminotransferase (ALT), Apoptosis regulator (Bax), Smad3, TGF-β, and NF-kB hepatic mRNA expression (using Real-time PCR), administration of Sil alone downregulated these expressions. Treatment with Vit E acid and/ or Cur along with Sil produced best results in this concern. B-cell lymphoma 2 (Bcl-2) expressions were downregulated by CCl 4 ; whereas concurrent treatment of Vit E and/or Cur along with Sil increased its expression. On conclusion, the use of Vit E and/or Cur could potentiate the antiapoptotic action of Sil. |
