Human heart failure biomarker immunosensor based on excessively tilted fiber gratings.

Autor: Luo B; Chongqing Key Laboratory of Optical Fiber Sensor and Photoelectric Detection, Chongqing University of Technology, Chongqing, 400054, China; School of Opto-electronic Information, University of Electronic Science and Technology of China, Chengdu, 610054, China; Aston Institute of Photonic Technologies, Aston University, Birmingham, B4 7ET, UK; luobinbin@cqut.edu.cn., Wu S; School of Pharmacy and Bioengineering, Chongqing University of Technology, Chongqing, 400054, China., Zhang Z; School of Pharmacy and Bioengineering, Chongqing University of Technology, Chongqing, 400054, China., Zou W; Chongqing Key Laboratory of Optical Fiber Sensor and Photoelectric Detection, Chongqing University of Technology, Chongqing, 400054, China., Shi S; Chongqing Key Laboratory of Optical Fiber Sensor and Photoelectric Detection, Chongqing University of Technology, Chongqing, 400054, China., Zhao M; Chongqing Key Laboratory of Optical Fiber Sensor and Photoelectric Detection, Chongqing University of Technology, Chongqing, 400054, China., Zhong N; Chongqing Key Laboratory of Optical Fiber Sensor and Photoelectric Detection, Chongqing University of Technology, Chongqing, 400054, China., Liu Y; School of Opto-electronic Information, University of Electronic Science and Technology of China, Chengdu, 610054, China., Zou X; Chongqing Key Laboratory of Optical Fiber Sensor and Photoelectric Detection, Chongqing University of Technology, Chongqing, 400054, China., Wang L; School of Pharmacy and Bioengineering, Chongqing University of Technology, Chongqing, 400054, China., Chai W; The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400050, China., Hu C; Department of Clinical Biochemistry, Third Military Medical University, Chongqing, 400038, China., Zhang L; Aston Institute of Photonic Technologies, Aston University, Birmingham, B4 7ET, UK.
Jazyk: angličtina
Zdroj: Biomedical optics express [Biomed Opt Express] 2016 Dec 05; Vol. 8 (1), pp. 57-67. Date of Electronic Publication: 2016 Dec 05 (Print Publication: 2017).
DOI: 10.1364/BOE.8.000057
Abstrakt: A label-free immunosensor platform based on excessively tilted fiber gratings (Ex-TFGs) was developed for highly specific and fast detection of human N-terminal pro-B-type natriuretic peptide (NT-proBNP), which is considered a powerful biomarker for prognosis and risk stratification of heart failure (HF). High-purity anti-NT-proBNP monoclonal antibodies (MAbs) prepared in our laboratory were immobilized on fiber surface through the staphylococcal protein A (SPA) method for subsequent specific binding of the targeted NT-proBNP. Utilizing fiber optic grating demodulation system (FOGDS), immunoassays were carried out in vitro by monitoring the resonance wavelength shift of Ex-TFG biosensor with immobilized anti-NT-proBNP MAbs. Lowest detectable concentration of ~0.5ng/mL for NT-proBNP was obtained, and average sensitivity for NT-proBNP at a concentration range of 0~1.0 ng/mL was approximately 45.967 pm/(ng/mL). Several human serum samples were assessed by the proposed Ex-TFG biomarker sensor, with high specificity for NT-proBNP, indicating potential application in early diagnosing patients with acute HF symptoms.
Databáze: MEDLINE