Hypermethylation of antisense long noncoding RNAs in acute lymphoblastic leukemia.
| Autor: | Arthur G; Department of Pathology & Anatomical Sciences, University of Missouri-Columbia, Columbia, MO 65212, USA.; MU Informatics Institute, University of Missouri-Columbia, Columbia, MO 65212, USA., Almamun M; Hematology/Oncology, Boston Children's Hospital, Harvard Medical School, Boston, MA 02115, USA., Taylor K; Department of Pathology & Anatomical Sciences, University of Missouri-Columbia, Columbia, MO 65212, USA. |
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| Jazyk: | angličtina |
| Zdroj: | Epigenomics [Epigenomics] 2017 May; Vol. 9 (5), pp. 635-645. Date of Electronic Publication: 2017 Jan 17. |
| DOI: | 10.2217/epi-2016-0156 |
| Abstrakt: | Aim: Long noncoding RNAs serve critical regulatory functions highly specific for a tissue and its developmental stage. Antisense long ncRNA (AS-lncRNA) methylation changes in acute lymphoblastic leukemia (ALL) versus normal pre-B-cell lymphoblasts were evaluated to identify potential differential methylation in this group of genes. Materials & Methods: The methylome of ALL and normal lymphoblasts was examined by the methylated CpG island recovery assay followed by NGS. Conclusion: The potential effect of trans regulation by AS-lncRNA through DNA/RNA binding is significant as sequence alignment analysis of the 25 most differentially methylated AS-lncRNAs revealed 368 genes containing highly similar sequences with a median nucleotide identity of 90.8% and binding span of 122 base pairs. Regulation of biological processes and anatomical structure development were over represented. ALL classification schemes based on AS-lncRNA methylation can provide new insights into its pathogenesis and treatment. |
| Databáze: | MEDLINE |
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