Immuno-PCR technology for detection of natural human antibodies against Le c disaccharide.

Autor: Maerle AV; JSC NPF DNA-Technology, Moscow, Russia. maerle@yandex.ru., Voronina DV; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia., Dobrochaeva KL; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia., Galanina OE; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia., Alekseev LP; NRC Institute of Immunology, Federal Biomedical Agency of Russia, Moscow, Russia., Bovin NV; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia., Zavriev SK; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia., Ryazantsev DY; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Jazyk: angličtina
Zdroj: Glycoconjugate journal [Glycoconj J] 2017 Apr; Vol. 34 (2), pp. 199-205. Date of Electronic Publication: 2017 Jan 14.
DOI: 10.1007/s10719-016-9751-6
Abstrakt: The development of an immuno-PCR assay for quantitation of low amounts of anti-glycan human antibodies is described. The sensitivity of the assay for determination of low-affinity anti-Le C IgM has been found to be 4 ng/ml (~100 pg per sample), thus being two orders of magnitude higher compared to the conventional ELISA with the same antigen.
Databáze: MEDLINE
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