| Autor: |
Porseva VV; Department of Normal Physiology and Biophysics, Yaroslavl State Medical University, Ministry of Health of the Russian Federation, Yaroslavl, Russia., Shilkin VV; Department of Normal Physiology and Biophysics, Yaroslavl State Medical University, Ministry of Health of the Russian Federation, Yaroslavl, Russia., Strelkov AA; Department of Normal Physiology and Biophysics, Yaroslavl State Medical University, Ministry of Health of the Russian Federation, Yaroslavl, Russia., Krasnov IB; Laboratory of Gravitational Biology, State Scientific Centre Institute of Biomedical Problems, Russian Academy of Sciences, Moscow, Russia., Masliukov PM; Department of Normal Physiology and Biophysics, Yaroslavl State Medical University, Ministry of Health of the Russian Federation, Yaroslavl, Russia. mpm@yma.ac.ru. |
| Abstrakt: |
Expression of choline acetyltransferase, 200-kDa neurofilament protein, 28-kDa calbindin, neuronal NO synthase, caspase 3, and Ki-67 in the motor neurons of spinal cord segments T3-T5 in male C57Bl/6 mice after 30-day space flight in the Bion-M1 biosatellite was studied by immunohistochemical methods. Under conditions space flight, the size of motoneurons increased, the number of neurons containing choline acetyltransferase and neurofilaments, decreased, and the number of calbindin-positive neurons increased; motoneurons, expressing neuronal NO synthase and caspase 3 appeared, while Ki-67 was not detected. Fragmentation of neurons with the formation structures similar to apoptotic (residual) bodies was observed in individual caspase 3-positive motoneurons. |
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