Neurogenin-2-transduced human neural progenitor cells attenuate neonatal hypoxic-ischemic brain injury.

Autor: Lee IS; Yonsei Biomedical Research Institute, Yonsei University College of Medicine, Seoul, Korea., Koo KY; Department of Pediatrics, Severance Children's Hospital, Yonsei University College of Medicine, Seoul, Korea., Jung K; Brain Korea 21 Plus Project for Medical Science, Yonsei University College of Medicine, Seoul, Korea., Kim M; Brain Korea 21 Plus Project for Medical Science, Yonsei University College of Medicine, Seoul, Korea., Kim IS; Yonsei Biomedical Research Institute, Yonsei University College of Medicine, Seoul, Korea., Hwang K; Brain Korea 21 Plus Project for Medical Science, Yonsei University College of Medicine, Seoul, Korea., Yun S; Brain Korea 21 Plus Project for Medical Science, Yonsei University College of Medicine, Seoul, Korea., Lee H; Yonsei Biomedical Research Institute, Yonsei University College of Medicine, Seoul, Korea., Shin JE; Department of Pediatrics, Severance Children's Hospital, Yonsei University College of Medicine, Seoul, Korea., Park KI; Yonsei Biomedical Research Institute, Yonsei University College of Medicine, Seoul, Korea; Department of Pediatrics, Severance Children's Hospital, Yonsei University College of Medicine, Seoul, Korea; Brain Korea 21 Plus Project for Medical Science, Yonsei University College of Medicine, Seoul, Korea. Electronic address: kipark@yuhs.ac.
Jazyk: angličtina
Zdroj: Translational research : the journal of laboratory and clinical medicine [Transl Res] 2017 May; Vol. 183, pp. 121-136.e9. Date of Electronic Publication: 2016 Dec 29.
DOI: 10.1016/j.trsl.2016.12.010
Abstrakt: Neonatal hypoxic-ischemic (HI) brain injury leads to high mortality and neurodevelopmental disabilities. Multipotent neural progenitor cells (NPCs) with self-renewing capacity have the potential to reduce neuronal loss and improve the compromised environment in the HI brain injury. However, the therapeutic efficacy of neuronal-committed progenitor cells and the underlying mechanisms of recovery are not yet fully understood. Therefore, this study investigated the regenerative ability and action mechanisms of neuronally committed human NPCs (hNPCs) transduced with neurogenin-2 (NEUROG2) in neonatal HI brain injury. NEUROG2- or green fluorescent protein (GFP)-encoding adenoviral vector-transduced hNPCs (NEUROG2- or GFP-NPCs) were transplanted into neonatal mouse brains with HI injury. Grafted NEUROG2-NPCs showed robust dispersion and engraftment, prolonged survival, and neuronal differentiation in HI brain injury. NEUROG2-NPCs significantly improved neurological behaviors, decreased cellular apoptosis, and increased the neurite outgrowth and axonal sprouting in HI brain injury. In contrast, GFP-NPC grafts moderately enhanced axonal extension with limited behavioral recovery. Notably, NEUROG2-NPCs showed increased secretion of multiple factors, such as nerve growth factor, brain-derived neurotrophic factor, neurotrophin-3 (NTF3), fibroblast growth factor 9 (FGF9), ciliary neurotrophic factor (CNTF), and thrombospondins 1 and 2 (THBS 1/2), which promoted SH-SY5Y neuroblastoma cell survival and neurite outgrowth. Thus, we postulate that NEUROG2-expressing human NPCs facilitate functional recovery after neonatal HI brain injury via their ability to secrete multiple factors that enhance neuronal survival and neuroplasticity.
(Copyright © 2016 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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