Energetic, oxidative and ionic exchange in rat brain and liver mitochondria at experimental audiogenic epilepsy (Krushinsky-Molodkina model).

Autor: Venediktova NI; Institute of Theoretical and Experimental Biophysics RAS, Pushchino, Moscow Region, 142290, Russia. tiggra4@yandex.ru., Gorbacheva OS; Institute of Theoretical and Experimental Biophysics RAS, Pushchino, Moscow Region, 142290, Russia., Belosludtseva NV; Institute of Theoretical and Experimental Biophysics RAS, Pushchino, Moscow Region, 142290, Russia., Fedotova IB; Biology Department, Laboratory for Physiology and Genetics of Behavior, Lomonosov Moscow State University, Leninskie Gory, 1, Build. 12, Moscow, 119992, Russia., Surina NM; Biology Department, Laboratory for Physiology and Genetics of Behavior, Lomonosov Moscow State University, Leninskie Gory, 1, Build. 12, Moscow, 119992, Russia., Poletaeva II; Biology Department, Laboratory for Physiology and Genetics of Behavior, Lomonosov Moscow State University, Leninskie Gory, 1, Build. 12, Moscow, 119992, Russia., Kolomytkin OV; Institute of Theoretical and Experimental Biophysics RAS, Pushchino, Moscow Region, 142290, Russia., Mironova GD; Institute of Theoretical and Experimental Biophysics RAS, Pushchino, Moscow Region, 142290, Russia.
Jazyk: angličtina
Zdroj: Journal of bioenergetics and biomembranes [J Bioenerg Biomembr] 2017 Apr; Vol. 49 (2), pp. 149-158. Date of Electronic Publication: 2017 Jan 09.
DOI: 10.1007/s10863-016-9693-5
Abstrakt: The role of brain and liver mitochondria at epileptic seizure was studied on Krushinsky-Molodkina (KM) rats which respond to sound with an intensive epileptic seizure (audiogenic epilepsy). We didn't find significant changes in respiration rats of brain and liver mitochondria of KM and control rats; however the efficiency of АТР synthesis in the KM rat mitochondria was 10% lower. In rats with audiogenic epilepsy the concentration of oxidative stress marker malondialdehyde in mitochondria of the brain (but not liver) was 2-fold higher than that in the control rats. The rate of H 2 O 2 generation in brain mitochondria of КМ rats was twofold higher than in the control animals when using NAD-dependent substrates. This difference was less pronounced in liver mitochondria. In KM rats, the activity of mitochondrial ATP-dependent potassium channel was lower than in liver mitochondria of control rats. The comparative study of the mitochondria ability to retain calcium ions revealed that in the case of using the complex I and complex II substrates, permeability transition pore is easier to trigger in brain and liver mitochondria of KM and КМs rats than in the control ones. The role of the changes in the energetic, oxidative, and ionic exchange in the mechanism of audiogenic epilepsy generation in rats and the possible correction of the epilepsy seizures are discussed.
Databáze: MEDLINE
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