Protective effect of Tritone (Livosone) on oxidative DNA damage and its hepatoprotective potential against various hepatotoxic agent in wistar rats.

Autor: Medhekar SK; Department of Pharmacology, Satara College of Pharmacy, Degaon, Satara, MH, India. Electronic address: smedhekar5@gmail.com., Jadhav TP; R & D center, Nisarga Biotech Pvt Ltd, Satara, MH, India. Electronic address: tejas.nisargabiotech@gmail.com., Sasane VS; R & D center, Nisarga Biotech Pvt Ltd, Satara, MH, India. Electronic address: vishal.nisargabiotech@gmail.com., Shende VS; Department of Pharmacology, Satara College of Pharmacy, Degaon, Satara, MH, India., Aloorkar NH; Department of Pharmaceutics, Satara College of Pharmacy, Degaon, Satara, MH, India., Chincholkar AB; Department of Pharmacology, Satara College of Pharmacy, Degaon, Satara, MH, India., Soman GS; R & D center, Nisarga Biotech Pvt Ltd, Satara, MH, India., Kulkarni AS; Department of Pharmaceutics, Satara College of Pharmacy, Degaon, Satara, MH, India.
Jazyk: angličtina
Zdroj: Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie [Exp Toxicol Pathol] 2017 Mar 02; Vol. 69 (3), pp. 153-161. Date of Electronic Publication: 2017 Jan 04.
DOI: 10.1016/j.etp.2016.12.005
Abstrakt: Aim: To evaluate antioxidant activity, DNA damage inhibition and hepatoprotecitve potential of polyherbal formulation Tritone (Livosone).
Methods: In vitro antioxidant activity of Tritone formulation was performed by using DPPH assay. Hepatoprotecitve potential of Tritone was evaluated against various hepatotoxic agents including Paracetamol (2g/kg b. wt p.o. single dose on 15th day), Galactosamine (400mg/kg b. wt. i.p. single dose on 8th day) and Alcohol (30% p.o.1ml/100g of rat for 15days). Tritone formulation at the doses of (40.5, 81 and 162mg/kg) and standard silymarin (100mg/kg) and Liv52 (270mg/kg) were administered p.o. The hepatoprotective assessment was done by estimating biochemical parameters: SGOT, SGPT, ALP and Total Bilirubin total protein and ChE levels. Additionally histopathological and DNA fragmentation study of Tritone was also performed.
Result: Administration of hepatotoxins (paracetamol, D-GaiN and alcohol) in experimental animals showed significant biochemical, histological deterioration and DNA fragmentation. Pretreatment with Tritone (Livosone) shows significant reduction in serum SGOT, SGPT, ALP and total bilirubin levels and shows significant elevation in total protein and cholinesterase (ChE) levels compared to groups treated with hepatotoxic agents. Histopathological observations of rat liver pretreated with Tritone (Livosone) shows significant protection against hepatic damage. Inhibition of DNA fragmentation by Tritone indicates protective effect of formulation on liver at molecular level. Finally all the results were compared with standard drugs Silymarin and Liv52.
Conclusion: Correlation of antioxidant activity, biochemical results, histopathological changes and inhibition of DNA damage after treatment with Tritone shows maximum hepatoprotective potential at dose 81mg/kg and 162mg/kg.
(Copyright © 2016 Elsevier GmbH. All rights reserved.)
Databáze: MEDLINE
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