Hapten-Specific T Cell-Mediated Skin Inflammation: Flow Cytometry Analysis of Mouse Skin Inflammatory Infiltrate.

Autor: Bouladoux N; Mucosal Immunology Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Disease, National Institutes of Health, Bethesda, MD, USA., Hennequin C; Centre d'Immunologie de Marseille-Luminy, Aix Marseille Université, Inserm, CNRS, Parc scientifique de Luminy, Case 906, 13288, Marseille Cedex 9, France.; DBV, 92120, Montrouge, France., Malosse C; Centre d'Immunologie de Marseille-Luminy, Aix Marseille Université, Inserm, CNRS, Parc scientifique de Luminy, Case 906, 13288, Marseille Cedex 9, France., Malissen B; Centre d'Immunologie de Marseille-Luminy, Aix Marseille Université, Inserm, CNRS, Parc scientifique de Luminy, Case 906, 13288, Marseille Cedex 9, France., Belkaid Y; Mucosal Immunology Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Disease, National Institutes of Health, Bethesda, MD, USA., Henri S; Centre d'Immunologie de Marseille-Luminy, Aix Marseille Université, Inserm, CNRS, Parc scientifique de Luminy, Case 906, 13288, Marseille Cedex 9, France. henri@ciml.univ-mrs.fr.
Jazyk: angličtina
Zdroj: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2017; Vol. 1559, pp. 21-36.
DOI: 10.1007/978-1-4939-6786-5_2
Abstrakt: Hapten-specific T cell-mediated skin inflammation also known as contact hypersensitivity (CHS) is characterized by a strong influx of CD8 + cytotoxic T cells within the skin upon reexposure of sensitized individuals to the same hapten. As many other leukocytes are also recruited during this elicitation phase, we attempted to revisit the skin infiltrate and characterize the inflammatory pattern. Recent improvement in the isolation in conventional as well as inflammatory dendritic cell and macrophage subsets from tissues and in the use of appropriate surface markers unraveling their heterogeneity should allow to determinate their specific functions in the CHS model. Here, we describe procedures to extract those cells from the skin and to analyze them by flow cytometry using a combination of appropriate surface markers allowing further transcriptomic analysis and functional assays.
Databáze: MEDLINE
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