Evaluation of P2X7 receptor expression in peripheral lymphocytes and immune profile from patients with indeterminate form of Chagas disease.

Autor: Souza VD; Department of Microbiology and Parasitology, Health Sciences Center, Federal University of Santa Maria, Santa Maria, RS 97105-900, Brazil., Dos Santos JT; Department of Large Animal Clinic de Clínica, Rural Sciences Center, Federal University of Santa Maria, Santa Maria, RS 97105-900, Brazil., Cabral FL; Department of Microbiology and Parasitology, Health Sciences Center, Federal University of Santa Maria, Santa Maria, RS 97105-900, Brazil., Barbisan F; Department of Morphology, Health Sciences Center, Federal University of Santa Maria, Santa Maria, RS 97105-900, Brazil., Azevedo MI; Department of Microbiology and Parasitology, Health Sciences Center, Federal University of Santa Maria, Santa Maria, RS 97105-900, Brazil., Dias Carli LF; Department of Microbiology and Parasitology, Health Sciences Center, Federal University of Santa Maria, Santa Maria, RS 97105-900, Brazil., de Avila Botton S; Department of Preventive Veterinary Medicine, Rural Sciences Center, Federal University of Santa Maria, Santa Maria, RS 97105-900, Brazil., Dos Santos Jaques JA; Center of Biological and Health Sciences, Federal University of Mato Grosso do Sul, Campo Grande, MS 79.070-900, Brazil., Rosa Leal DB; Department of Microbiology and Parasitology, Health Sciences Center, Federal University of Santa Maria, Santa Maria, RS 97105-900, Brazil. Electronic address: dbitencourtrosaleal@gmail.com.
Jazyk: angličtina
Zdroj: Microbial pathogenesis [Microb Pathog] 2017 Mar; Vol. 104, pp. 32-38. Date of Electronic Publication: 2017 Jan 03.
DOI: 10.1016/j.micpath.2017.01.002
Abstrakt: Chagas disease (CD) is caused by Trypanosoma cruzi, an intracellular protozoan which is a potent stimulator of cell-mediated immunity. In the indeterminate form of CD (IFCD) a modulation between pro- and anti-inflammatory responses establishes a host-parasite adaptation. It was previously demonstrated that purinergic ecto-enzymes regulates extracellular ATP and adenosine levels, influencing immune and inflammatory processes during IFCD. In inflammatory sites ATP, as well as its degradation product, adenosine, function as signaling molecules and immunoregulators through the activation of purinergic receptors. In this work, it was analyzed the gene and protein expression of P2X7 purinergic receptor in peripheral lymphocytes and serum immunoregulatory cytokines from IFCD patients. Gene and protein expression of P2X7 receptor (P2X7R), and serum cytokines (IL-2, IL-10, IL-17 and IFN-γ) were unaltered. However, IFCD group showed significantly higher IL-4 and IL-6 levels while TNF-α was significantly decreased. These results indicate that imune profile of IFCD patients displays anti-inflammatory characteristics, consistent with the establishment of an immunomodulatory response. Further study about the molecular knowledge of P2X7R in IFCD is useful to clarify the participation of purinergic system in the regulatory mechanism which avoid the progression of CD.
(Copyright © 2017 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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