Quantification of two isomeric flavones in rat colon tissue using reverse phase high performance liquid chromatography.
| Autor: | Whitted CL; Department of Pharmaceutical Sciences, Gatton College of Pharmacy, East Tennessee State University, Box 70594, Johnson City, TN, 37614-1708, USA., Palau VE; Department of Pharmaceutical Sciences, Gatton College of Pharmacy, East Tennessee State University, Box 70594, Johnson City, TN, 37614-1708, USA., Torrenegra RD; Universidad de Ciencias Aplicadas y Ambientales, Bogotá, Colombia., Rodriguez OE; Department of Environmental Engineering, Faculty of Engineering, Universidad El Bosque, Bogotá, Colombia., Harirforoosh S; Department of Pharmaceutical Sciences, Gatton College of Pharmacy, East Tennessee State University, Box 70594, Johnson City, TN, 37614-1708, USA. harirfor@etsu.edu. |
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| Jazyk: | angličtina |
| Zdroj: | BMC research notes [BMC Res Notes] 2017 Jan 07; Vol. 10 (1), pp. 29. Date of Electronic Publication: 2017 Jan 07. |
| DOI: | 10.1186/s13104-016-2358-y |
| Abstrakt: | Background: Antineoplastic activity has been previously shown for two isomeric flavones, 5,7-dihydroxy-3,6,8-trimethoxy flavone (flavone A) and 3,5-dihydroxy-6,7,8-trimethoxy flavone (flavone B), against colon cancer cell lines (Thomas et al. in PLoS ONE 7:e39806, 5). Here, we present modified methods for the extraction and quantification of flavones A and B in rat colon tissue after intravenous dosing via high performance liquid chromatography, from the originally described procedure for extraction and quantification in rat plasma (Whitted et al. in J Chromatogr B Analyt Technol Biomed Life Sci 1001:150-155, 7). Results: Modifications included tissue homogenization (1 g tissue: 2 mL water), filtration of the supernatant with a PVDF membrane, and the use of only one calibration curve to determine the concentration of each flavone in colon tissue. Good separation was achieved and representative equations were linear with r 2 ≥ 0.99 for both flavones. Precision and accuracy for flavone A ranged from 0.88-24.03 and 109-116%. Precision and accuracy for flavone B ranged from 1.62-33.56 and 98-113%. Concentrations of 1639 ± 601 ng/g flavone A and 5975 ± 2480 ng/g of flavone B were detected in rat colon tissue 6 h post dosing. Conclusions: Modifications to the extraction methods for flavone A and flavone B from rat colon tissue had good separation, precision, and accuracy. |
| Databáze: | MEDLINE |
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