Polarization microscopy as a tool for quantitative evaluation of collagen using picrosirius red in different stages of CKD in cats.

Autor:	Morais GB; Faculdade de Veterinária, Universidade Estadual do Ceará, Ceará, Brazil., Viana DA; Faculdade de Veterinária, Universidade Estadual do Ceará, Ceará, Brazil., Silva FMO; Faculdade de Veterinária, Universidade Estadual do Ceará, Ceará, Brazil., Xavier Júnior FAF; Faculdade de Veterinária, Universidade Estadual do Ceará, Ceará, Brazil., Farias KM; Departamento de Fisiologia e Farmacologia, Universidade Federal do Ceará, Ceará, Brazil., Pessoa CD; Departamento de Fisiologia e Farmacologia, Universidade Federal do Ceará, Ceará, Brazil., Silveira JAM; Departamento de Fisiologia e Farmacologia, Universidade Federal do Ceará, Ceará, Brazil., Alves APNN; Faculdade de Farmácia Odontologia e Enfermagem, Universidade Federal do Ceará, Ceará, Brazil., Mota MRL; Faculdade de Farmácia Odontologia e Enfermagem, Universidade Federal do Ceará, Ceará, Brazil., Silva FDO; Departamento de Geologia, Universidade Federal do Ceará, Ceará, Brazil., Sampaio CMS; Centro de Ciências da Saúde, Universidade Estadual do Ceará, Ceará, Brazil., Verdugo JMG; Instituto Cavanilles Universidad de Valencia, Valencia, Brazil., Evangelista JSAM; Faculdade de Veterinária, Universidade Estadual do Ceará, Ceará, Brazil.
Jazyk:	angličtina
Zdroj:	Microscopy research and technique [Microsc Res Tech] 2017 May; Vol. 80 (5), pp. 543-550. Date of Electronic Publication: 2017 Jan 06.
DOI:	10.1002/jemt.22830
Abstrakt:	Chronic kidney disease (CKD) is a relevant disease in feline clinic. The tubulointerstitial damage, with collagen deposition and fibrosis, is an important result of this process. The aim of this study was to quantify and correlate the deposition of collagen and severity of interstitial fibrosis (IF) in the kidney from cats in different stages of CKD. Kidney fragments from 10 adult cats with CKD were analyzed and stained by Masson's trichrome (MT) and Picrosirius red (PSR) for circular polarized microscopy. Random quantitative analysis was performed on MT sections to classify the degree of IF, per field area, with and without circular polarization. Statistics correlations were performed by Spearman's (ρ; p < .05). There was a significant correlation of IF quantification with the area of interstitial collagen deposition by polarized PSR (PSRp) (r = .7939, p = .0098) and nonpolarized PSR (PSRn) (r = .7781, p = .0080). There was a positive correlation of serum creatinine (sCr) at different stages of CKD with PSRp (r = .7939, p = .0098), PSRn (r = .8667, p = .0027) and MT (r = .7818, p = .0117). Correlations between the percentage of quantified area was also positive from PSRp to PSRn (r = .9030, p = .0009) and PSRp to MT (r = .7939, p = .0098). The PSRN was also correlated with MT (r = .9273, p = .0001). The correlation with IF and sCr follows the disease evolution and the quantification of collagen by PSR is an excellent tool for analyzing the disease severity at different stages. (© 2016 Wiley Periodicals, Inc.)
Databáze:	MEDLINE
Externí odkaz:	Plný text