Heterogeneity in Allergy to Mollusks: A Clinical-Immunological Study in a Population From the North of Spain.

Autor:	Azofra J; Sección de Alergia, Área de Gestión del Pulmón, Hospital Universitario Central de Asturias, Oviedo, Spain., Echechipía S; Servicio de Alergia, Complejo Hospitalario de Navarra, Pamplona, Spain., Irazábal B; Servicio de Alergia, Hospital de Cruces, Bilbao, Spain., Muñoz D; Servicio de Alergia, Hospital Universitario de Araba, Vitoria, Spain., Bernedo N; Servicio de Alergia, Hospital Universitario de Araba, Vitoria, Spain., García BE; Servicio de Alergia, Complejo Hospitalario de Navarra, Pamplona, Spain., Gastaminza G; Departamento de Alergia, Clínica Universidad de Navarra, Pamplona, Spain., Goikoetxea MJ; Departamento de Alergia, Clínica Universidad de Navarra, Pamplona, Spain., Joral A; Servicio de Alergia, Hospital Donostia, San Sebastián, Spain., Lasa E; Servicio de Alergia, Hospital Donostia, San Sebastián, Spain., Gamboa P; Servicio de Alergia, Hospital de Basurto, Bilbao, Spain., Díaz C; Sección de Alergia, Área de Gestión del Pulmón, Hospital Universitario Central de Asturias, Oviedo, Spain., Beristain A; Sección de Alergia, Área de Gestión del Pulmón, Hospital Universitario Central de Asturias, Oviedo, Spain., Quiñones D; Sección de Alergia, Área de Gestión del Pulmón, Hospital Universitario Central de Asturias, Oviedo, Spain., Bernaola G; Servicio de Alergia, Hospital de Galdácano, Bilbao, Spain., Echenagusia MA; Servicio de Alergia, Hospital de Mendaro, Guipúzcoa, Spain., Liarte I; Servicio de Alergia, Hospital de Cruces, Bilbao, Spain., García E; Servicio de Alergia, Hospital de Basurto, Bilbao, Spain., Cuesta J; Departamento de Alergia, Fundación Jiménez Díaz, Madrid, Spain., Martínez MD; Servicio de Alergia, Hospital de Cruces, Bilbao, Spain., Velasco M; Servicio de Alergia, Hospital Universitario de Araba, Vitoria, Spain., Longo N; Servicio de Alergia, Hospital Universitario de Araba, Vitoria, Spain., Pastor-Vargas C; Departamento de Inmunología, IIS Fundación Jiménez Díaz, UAM, Madrid, Spain.
Jazyk:	angličtina
Zdroj:	Journal of investigational allergology & clinical immunology [J Investig Allergol Clin Immunol] 2024 Oct 01; Vol. 27 (4), pp. 252-260. Date of Electronic Publication: 2017 Jan 03.
DOI:	10.18176/jiaci.0137
Abstrakt:	Background and Objective: Allergy to mollusks has been the focus of fewer studies than allergy to crustaceans. Furthermore, allergy to mollusks is less well characterized. Objectives: To describe the clinical characteristics of mollusk-allergic patients, to identify the responsible allergens, and to assess cross-reactivity. Methods: We performed a prospective multicenter study including 45 patients with mollusk allergy, which was diagnosed based on a suggestive clinical history and a positive skin test result with the agent involved. Fractions were identified using SDS-PAGE and immunoblotting. The proteins responsible were subsequently identified using mass spectrometry. ELISA inhibition studies were performed with mollusks, dust mites, and crustaceans. Results: We found that 25 patients (55%) were allergic to cephalopods, 14 (31%) to bivalves, and 11 (24%) to gastropods. Limpet was the third most frequent cause of allergy (15% of cases). In 31 patients (69%), the manifestation was systemic; 10 (22%) exhibited oral allergy syndrome, and 7 (15%) experienced contact urticaria. Most major allergens were found between 27 kDa and 47 kDa. ELISA inhibition assays revealed a high degree of inhibition of cephalopods and bivalves from all the groups of mollusks, mites, and crustaceans. Mass spectrometry identified tropomyosin, actin, and myosin as the major allergens. Conclusions: Cephalopods, especially squid, are the mollusks that most frequently trigger allergic symptoms. The very frequent occurrence of allergy to limpets is striking, given their low consumption in our area. It is worth highlighting the heterogeneity observed, exemplified by the gastropods. Tropomyosin appears to be responsible for the high cross-reactivity found between mollusks, mites, and crustaceans. Three new mollusk allergens were also identified, namely, actin, enolase, and a putative C1q domain-containing protein.
Databáze:	MEDLINE
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