| Autor: |
Kesavan MP; Chemistry Research Centre, Mohamed Sathak Engineering College, Kilakarai 623 806, Tamilnadu, India; Department of Chemistry, Sethu Institute of Technology, Pulloor, Kariapatti 626 115, Tamilnadu, India., Vinoth Kumar GG; Chemistry Research Centre, Mohamed Sathak Engineering College, Kilakarai 623 806, Tamilnadu, India; Department of Chemistry, Sethu Institute of Technology, Pulloor, Kariapatti 626 115, Tamilnadu, India., Dhaveethu Raja J; Chemistry Research Centre, Mohamed Sathak Engineering College, Kilakarai 623 806, Tamilnadu, India., Anitha K; School of Physics, Madurai Kamaraj University, Madurai 625 021, Tamilnadu, India., Karthikeyan S; Department of Microbiology, Sourashtra College, Madurai 625 004, Tamilnadu, India., Rajesh J; Chemistry Research Centre, Mohamed Sathak Engineering College, Kilakarai 623 806, Tamilnadu, India; Department of Chemistry, Sethu Institute of Technology, Pulloor, Kariapatti 626 115, Tamilnadu, India. Electronic address: mkuraji@gmail.com. |
| Abstrakt: |
Luotonin A (L), a novel natural cytotoxic and anti-inflammatory alkaloid, chelated with copper(II) to improve its cytotoxic effect against the cancer cells. The complexes [Cu(L)H 2 OCl]Cl (1) and [Cu(L) 2 ]Cl 2 (2) are prepared by using copper(II) chloride and L with ligand/metal molar ratio of 1:1 and 2:1 respectively. A solution of complexes 1 &2 are characterized by physical spectroscopic methods using Ultraviolet-visible (UV-Vis) spectrophotometer, Fourier Transform-Infra red (FT-IR) spectroscopy, Electron Para magnetic Resonance Spectroscopy (EPR) and by electrochemical methods. The interaction of these complexes 1 &2 with calf thymus (CT-DNA) have been investigated by physical methods to propose the modes of DNA binding with the complexes 1 &2. Absorption spectral titration studies of complex 1 with CT-DNA shows a red-shift of 5nm with the DNA binding affinity of K b , 8.65×10 3 M -1 , but complex 2does not show any red-shift with binding constant K b , 7.32×10 3 M -1 reveals that the complex 1 binding with DNA strongly than complex 2 and the binding occurs in between the base pairs of DNA as intercalation. Strong interactions of the two complexes 1 & 2 with CT-DNA have also been confirmed by fluorescence spectral titration studies. The evaluated values of K SV and K ass shows that, the complexes 1 &2 interact with DNA through the intercalation, coincide with other partial intercalators strongly than the free ligand L. Complex 1 exhibits potent antioxidant activity with SC 50 value of 23.9±0.69μM is evaluated by DPPH radical scavenging assay and which has potent antimicrobial activity against pathogens than 2 and L. The anticancer activity of L, complexes 1 &2 against human breast cancer cell line (MCF-7) and cervical cancer cell line (HeLa) has also been studied by using fluorescence staining method. The IC 50 values of L, complexes 1&2 against MCF-7 and HeLa cell lines with the incubation time intervals of 24hrs are 1 (5.0±0.25, 12.0±0.30μM)<2 (6.5±0.27, 15.0±0.30μM) (Copyright © 2016 Elsevier B.V. All rights reserved.) |
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