| Autor: |
Sitarek P; Department of Biology and Pharmaceutical Botany, Medical University of Lodz, Muszyńskiego Street 1, 90-151, Łódź, Poland. przemyslaw.sitarek@umed.lodz.pl., Skała E; Department of Biology and Pharmaceutical Botany, Medical University of Lodz, Muszyńskiego Street 1, 90-151, Łódź, Poland., Toma M; Department of Molecular Genetics, University of Lodz, Łódź, Poland., Wielanek M; Department of Plant Physiology and Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Łódź, Poland., Szemraj J; Department of Medical Biochemistry, Medical University of Lodz, Łódź, Poland., Skorski T; Department of Microbiology and Immunology, and Fels Institute for Cancer Research, School of Medicine, Temple University, Philadelphia, PA, USA., Białas AJ; Department of Pneumology and Allergy, 1st Chair of Internal Medicine, Medical University of Lodz, Łódź, Poland., Sakowicz T; Department of Genetics and Plant Molecular Biology and Biotechnology, The University of Lodz, Łódź, Poland., Kowalczyk T; Department of Genetics and Plant Molecular Biology and Biotechnology, The University of Lodz, Łódź, Poland., Radek M; Department of Neurosurgery, Surgery of Spine and Peripheral Nerves, Medical University of Łódź, University Hospital WAM-CSW, Łódź, Poland., Wysokińska H; Department of Biology and Pharmaceutical Botany, Medical University of Lodz, Muszyńskiego Street 1, 90-151, Łódź, Poland., Śliwiński T; Department of Molecular Genetics, University of Lodz, Łódź, Poland. |
| Abstrakt: |
This study determines the influence of transformed root (TR) extract of Leonurus sibiricus L. on various grades (I-III) of human glioma cells derived from patients. This plant occurs in southern Asia and Siberia and is widely used as a medicinal plant with various biological activities. Chromatographic profile of TR extract have revealed the presence of various polyphenolic compounds (4-hydroxybenzoic acid, gentisic acid, vanilic acid, 1,3-dicaffeoylquinic acid, α-resorcylic acid). We found TR root extract to have antiproliferative activity on glioma cells after 24 h of treatment. TR root extract induces apoptosis on various grades (I-III) of human glioma cells by the generation of reactive oxygen species (ROS) along with concurrent loss of mitochondrial membrane potential, enhanced S and G2/M phases of the cell cycle, and altered mRNA levels of Bax, Bcl-2, p53, Cas-3, Cas-8 and Cas-9 factors involved in apoptosis. This work for the first time demonstrate that TR extract from L. sibiricus root has the potential to activate apoptosis in grade I-III human glioma cells through the intrinsic and extrinsic pathways. |
Full text from SpringerLink