Tooth tissue engineering: tooth decellularization for natural scaffold.

Autor:	de Sousa Iwamoto LA; CTCMol, Center of Cellular & Molecular Therapy, UNIFESP- Universidade Federal de Sao Paulo- Escola Paulista de Medicina, Sao Paulo, Brazil; Translational Surgery, Surgery Department, UNIFESP- Universidade Federal de Sao Paulo- Escola Paulista de Medicina, Sao Paulo, Brazil; CTCMol, Center of Cellular & Molecular Therapy, UNIFESP- Universidade Federal de Sao Paulo- Escola Paulista de Medicina, Sao Paulo, Brazil; Translational Surgery, Surgery Department, UNIFESP- Universidade Federal de Sao Paulo- Escola Paulista de Medicina, Sao Paulo, Brazil., Duailibi MT; CTCMol, Center of Cellular & Molecular Therapy, UNIFESP- Universidade Federal de Sao Paulo- Escola Paulista de Medicina, Sao Paulo, Brazil; Translational Surgery, Surgery Department, UNIFESP- Universidade Federal de Sao Paulo- Escola Paulista de Medicina, Sao Paulo, Brazil; National Institute of Science & Technology, Biofabrication Institute, BIOFABRIS, Campinas, São Paulo, Brazil; CTCMol, Center of Cellular & Molecular Therapy, UNIFESP- Universidade Federal de Sao Paulo- Escola Paulista de Medicina, Sao Paulo, Brazil; Translational Surgery, Surgery Department, UNIFESP- Universidade Federal de Sao Paulo- Escola Paulista de Medicina, Sao Paulo, Brazil; National Institute of Science & Technology, Biofabrication Institute, BIOFABRIS, Campinas, São Paulo, Brazil., Iwamoto GY; Material Engineering, UNIFESP- Universidade Federal de São Paulo, Sao José dos Campos, Sao Paulo, Brazil; Material Engineering, UNIFESP- Universidade Federal de São Paulo, Sao José dos Campos, Sao Paulo, Brazil., Juliano Y; Health Science Department, UNISA - Universidade de Santo Amaro, Sao Paulo, Brazil; Health Science Department, UNISA - Universidade de Santo Amaro, Sao Paulo, Brazil., Duailibi MS; Faculdade de Ciencias Medicas Santa Casa de Sao Paulo, Sao Paulo, Brazil; Faculdade de Ciencias Medicas Santa Casa de Sao Paulo, Sao Paulo, Brazil., Ossamu Tanaka FA; ESALQ- Escola Superior de Agronomia Luiz de Queiroz, Universidade de Sao Paulo, Sao Paulo, Brazil; ESALQ- Escola Superior de Agronomia Luiz de Queiroz, Universidade de Sao Paulo, Sao Paulo, Brazil., Duailibi SE; CTCMol, Center of Cellular & Molecular Therapy, UNIFESP- Universidade Federal de Sao Paulo- Escola Paulista de Medicina, Sao Paulo, Brazil; Translational Surgery, Surgery Department, UNIFESP- Universidade Federal de Sao Paulo- Escola Paulista de Medicina, Sao Paulo, Brazil; National Institute of Science & Technology, Biofabrication Institute, BIOFABRIS, Campinas, São Paulo, Brazil; CTCMol, Center of Cellular & Molecular Therapy, UNIFESP- Universidade Federal de Sao Paulo- Escola Paulista de Medicina, Sao Paulo, Brazil; Translational Surgery, Surgery Department, UNIFESP- Universidade Federal de Sao Paulo- Escola Paulista de Medicina, Sao Paulo, Brazil; National Institute of Science & Technology, Biofabrication Institute, BIOFABRIS, Campinas, São Paulo, Brazil.
Jazyk:	angličtina
Zdroj:	Future science OA [Future Sci OA] 2016 Mar 31; Vol. 2 (2), pp. FSO121. Date of Electronic Publication: 2016 Mar 31 (Print Publication: 2016).
DOI:	10.4155/fsoa-2016-0016
Abstrakt:	Aim: Tissue engineering is a multidisciplinary science that aims to produce replacement organs and biological substitutes. One of the techniques involves decellularizing a biological organ without altering its structure. One challenge is how to demonstrate which method would be better for this process. Methodology: Fifty premolar teeth were divided into five groups: G1 (control): solution of 10% formaldehyde; G2: phosphate buffer saline (PBS), 28 g of tetrasodium ethylenediaminetetraacetic (EDTA), sodium hypochlorite 2.5% (SH); G3: PBS, EDTA and 40v hydrogen peroxide (HP); G4: PBS, EDTA, SH, enzymatic detergent (ED); and G5: PBS, EDTA, HP, ED. Each group was analyzed by scanning electron microscopy (SEM), x-ray, measured weights and color and received statistical analysis. Conclusion: This study demonstrated that G5 was the most appropriate method to obtain a natural scaffold. Competing Interests: Financial & competing interests disclosure The authors have no relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript. This includes employment, consultancies, honoraria, stock ownership or options, expert testimony, grants or patents received or pending, or royalties. No writing assistance was utilized in the production of this manuscript.
Databáze:	MEDLINE
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