Presence of environmental coagulase-positive staphylococci, their clonal relationship, resistance factors and ability to form biofilm.

Autor:	Velázquez-Guadarrama N; Laboratorio de Infectología, Hospital Infantil de México Federico Gómez, México, DF 06720, Mexico. Electronic address: normave@himfg.edu.mx., Olivares-Cervantes AL; Laboratorio de Infectología, Hospital Infantil de México Federico Gómez, México, DF 06720, Mexico., Salinas E; Centro de Ciencias de la Atmosfera, Universidad Nacional Autónoma de México, México, DF 04510, Mexico., Martínez L; Centro de Ciencias de la Atmosfera, Universidad Nacional Autónoma de México, México, DF 04510, Mexico., Escorcia M; Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, México, DF 04510, Mexico., Oropeza R; Instituto de Biotecnología Universidad Nacional Autónoma de México, Cuernavaca Mor, Mexico., Rosas I; Centro de Ciencias de la Atmosfera, Universidad Nacional Autónoma de México, México, DF 04510, Mexico. Electronic address: iarp@atmosfera.unam.mx.
Jazyk:	angličtina
Zdroj:	Revista Argentina de microbiologia [Rev Argent Microbiol] 2017 Jan - Mar; Vol. 49 (1), pp. 15-23. Date of Electronic Publication: 2016 Dec 23.
DOI:	10.1016/j.ram.2016.08.006
Abstrakt:	Coagulase-positive staphylococci (CoPS) are opportunistic pathogens carrying various mechanisms of resistance that have a large number of virulence factors, and whose ability to induce illness is associated with the host. This study aimed to investigate the presence of environmental coagulase-positive staphylococci, their susceptibility profile, clonal relationship and ability to form biofilm. The 16S rRNA genes from CoPS isolates were analyzed, and their antibiotic susceptibility was evaluated using the agar dilution method in accordance with Clinical and Laboratory Standards Institute guidelines. The clonal profile was obtained by pulsed-field gel electrophoresis (PFGE) and biofilm formation was measured by a crystal violet retention assay. A total of 72 Staphylococcus spp. strains were isolated from air, metal surfaces, and nostrils from humans, dogs, cats, and birds. Three species were identified: Staphylococcus aureus (17%), Staphylococcus intermedius (63%), and Staphylococcus pseudintermedius (21%). Ninety three percent (93%) of the strains were resistant to at least one of 13 tested antibiotics. S. pseudintermedius strains were the only resistant ones to methicillin while most of these isolates were multidrug-resistant, had significantly higher ability to form biofilm and PFGE grouped into seven different patterns, without showing clonal dispersion among animals and environmental isolates. This study suggests that dogs, cat, and air are environmental sources potentially carrying multidrug-resistant S. pseudintermedius, which survives in different environments through biofilm formation and multidrug resistance, characteristics that can be transmitted horizontally to other bacteria and exacerbate the problem of antibiotic resistance in humans. (Copyright © 2016 Asociación Argentina de Microbiología. Publicado por Elsevier España, S.L.U. All rights reserved.)
Databáze:	MEDLINE
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