Ebola virus VP35 blocks stress granule assembly.

Autor: Le Sage V; HIV-1 RNA Trafficking Laboratory, Lady Davis Institute at the Jewish General Hospital, Montréal, Québec, Canada H3T 1E2., Cinti A; HIV-1 RNA Trafficking Laboratory, Lady Davis Institute at the Jewish General Hospital, Montréal, Québec, Canada H3T 1E2; Department of Medicine, McGill University, Montréal, Québec, Canada H3A 0G4., McCarthy S; Centre for Innovation, Canadian Blood Services, Toronto, Ontario, Canada; Department of Laboratory Medicine and Pathobiology, University of Toronto, Ontario, Toronto, Canada., Amorim R; HIV-1 RNA Trafficking Laboratory, Lady Davis Institute at the Jewish General Hospital, Montréal, Québec, Canada H3T 1E2; Department of Medicine, McGill University, Montréal, Québec, Canada H3A 0G4., Rao S; HIV-1 RNA Trafficking Laboratory, Lady Davis Institute at the Jewish General Hospital, Montréal, Québec, Canada H3T 1E2; Department of Microbiology and Immunology, McGill University, Montréal, Québec, Canada H3A 0G4., Daino GL; Department of Life and Environmental Sciences, University of Cagliari, Cittadella Universitaria di Monserrato, SS 554, 09042 Monserrato, Cagliari, Italy., Tramontano E; Department of Life and Environmental Sciences, University of Cagliari, Cittadella Universitaria di Monserrato, SS 554, 09042 Monserrato, Cagliari, Italy., Branch DR; Centre for Innovation, Canadian Blood Services, Toronto, Ontario, Canada; Department of Laboratory Medicine and Pathobiology, University of Toronto, Ontario, Toronto, Canada., Mouland AJ; HIV-1 RNA Trafficking Laboratory, Lady Davis Institute at the Jewish General Hospital, Montréal, Québec, Canada H3T 1E2; Department of Medicine, McGill University, Montréal, Québec, Canada H3A 0G4; Department of Microbiology and Immunology, McGill University, Montréal, Québec, Canada H3A 0G4. Electronic address: andrew.mouland@mcgill.ca.
Jazyk: angličtina
Zdroj: Virology [Virology] 2017 Feb; Vol. 502, pp. 73-83. Date of Electronic Publication: 2016 Dec 23.
DOI: 10.1016/j.virol.2016.12.012
Abstrakt: Stress granules (SGs) are dynamic cytoplasmic aggregates of translationally silenced mRNAs that assemble in response to environmental stress. SGs appear to play an important role in antiviral innate immunity and many viruses have evolved to block or subvert SGs components for their own benefit. Here, we demonstrate that intracellular Ebola virus (EBOV) replication and transcription-competent virus like particles (trVLP) infection does not lead to SG assembly but leads to a blockade to Arsenite-induced SG assembly. Moreover we show that EBOV VP35 represses the assembly of canonical and non-canonical SGs induced by a variety of pharmacological stresses. This SG blockade requires, at least in part, the C-terminal domain of VP35. Furthermore, results from our co-immunoprecipitation studies indicate that VP35 interacts with multiple SG components, including G3BP1, eIF3 and eEF2 through a stress- and RNA-independent mechanism. These data suggest a novel function for EBOV VP35 in the repression of SG assembly.
(Copyright © 2016 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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