Emphysema induced by elastase enhances acute inflammatory pulmonary response to intraperitoneal LPS in rats.

Autor: da Fonseca LM; Pulmonary Research Laboratory, Universidade Federal de Juiz de Fora, Juiz de Fora, Brazil.; Center of Reproductive Biology, Universidade Federal de Juiz de Fora, Juiz de Fora, Brazil., Reboredo MM; Pulmonary Research Laboratory, Universidade Federal de Juiz de Fora, Juiz de Fora, Brazil.; Center of Reproductive Biology, Universidade Federal de Juiz de Fora, Juiz de Fora, Brazil., Lucinda LM; Pulmonary Research Laboratory, Universidade Federal de Juiz de Fora, Juiz de Fora, Brazil.; Center of Reproductive Biology, Universidade Federal de Juiz de Fora, Juiz de Fora, Brazil.; Department of Morphology, Institute of Biological Sciences, Universidade Federal de Juiz de Fora, Juiz de Fora, Brazil., Fazza TF; Pulmonary Research Laboratory, Universidade Federal de Juiz de Fora, Juiz de Fora, Brazil.; Center of Reproductive Biology, Universidade Federal de Juiz de Fora, Juiz de Fora, Brazil., Rabelo MA; Pulmonary Research Laboratory, Universidade Federal de Juiz de Fora, Juiz de Fora, Brazil.; Department of Morphology, Institute of Biological Sciences, Universidade Federal de Juiz de Fora, Juiz de Fora, Brazil., Fonseca AS; Department of Biophysics and Biometry, Institute of Biology Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, Juiz de Fora, Brazil., de Paoli F; Department of Morphology, Institute of Biological Sciences, Universidade Federal de Juiz de Fora, Juiz de Fora, Brazil., Pinheiro BV; Pulmonary Research Laboratory, Universidade Federal de Juiz de Fora, Juiz de Fora, Brazil.; Center of Reproductive Biology, Universidade Federal de Juiz de Fora, Juiz de Fora, Brazil.
Jazyk: angličtina
Zdroj: International journal of experimental pathology [Int J Exp Pathol] 2016 Dec; Vol. 97 (6), pp. 430-437. Date of Electronic Publication: 2016 Dec 23.
DOI: 10.1111/iep.12214
Abstrakt: Abnormalities in lungs caused by emphysema might alter their response to sepsis and the occurrence of acute lung injury (ALI). This study compared the extension of ALI in response to intraperitoneal lipopolysaccharide (LPS) injection in Wistar rats with and without emphysema induced by elastase. Adult male Wistar rats were randomized into four groups: control, emphysema without sepsis, normal lung with sepsis and emphysema with sepsis. Sepsis was induced, and 24 h later the rats were euthanised. The following analysis was performed: blood gas measurements, bronchoalveolar lavage (BAL), lung permeability and histology. Animals that received LPS showed significant increase in a lung injury scoring system, inflammatory cells in bronchoalveolar lavage (BAL) and IL-6, TNF-α and CXCL2 mRNA expression in lung tissue. Animals with emphysema and sepsis showed increased alveolocapillary membrane permeability, demonstrated by higher BAL/serum albumin ratio. In conclusion, the presence of emphysema induced by elastase increases the inflammatory response in the lungs to a systemic stimulus, represented in this model by the intraperitoneal injection of LPS.
(© 2016 The Authors. International Journal of Experimental Pathology © 2016 International Journal of Experimental Pathology.)
Databáze: MEDLINE
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