Circulating cytokeratin-positive cells and tumor budding in colorectal cancer.

Autor:	Märkl B; Bruno Märkl, Narjes Wilhelms, Institute of Pathology, Klinikum Augsburg, 86156 Augsburg, Germany., Wilhelms N; Bruno Märkl, Narjes Wilhelms, Institute of Pathology, Klinikum Augsburg, 86156 Augsburg, Germany., Anthuber M; Bruno Märkl, Narjes Wilhelms, Institute of Pathology, Klinikum Augsburg, 86156 Augsburg, Germany., Schenkirsch G; Bruno Märkl, Narjes Wilhelms, Institute of Pathology, Klinikum Augsburg, 86156 Augsburg, Germany., Schlimok G; Bruno Märkl, Narjes Wilhelms, Institute of Pathology, Klinikum Augsburg, 86156 Augsburg, Germany., Oruzio D; Bruno Märkl, Narjes Wilhelms, Institute of Pathology, Klinikum Augsburg, 86156 Augsburg, Germany.
Jazyk:	angličtina
Zdroj:	World journal of clinical oncology [World J Clin Oncol] 2016 Dec 10; Vol. 7 (6), pp. 433-440.
DOI:	10.5306/wjco.v7.i6.433
Abstrakt:	Aim: To investigate whether circulating cytokeratin-positive (CK + ) cells in the mesenteric blood of resected colorectal specimens are prognostic and correlate with tumor budding. Methods: Fifty-six colorectal specimens were collected between 9/2007 and 7/2008. Blood from the mesenteric vein was drawn immediately after receiving the fresh and unfixed specimens in the pathology department. After separation of the mononuclear cells by Ficoll-Hypaque density-gradient centrifugation, cytological smears were immunocytochemically stained for CK18. Tumor budding was evaluated on slides stained for pan-cytokeratin. The identification of ≥ 30 buds/1.3 mm 2 was defined as high grade budding. Results: CK + cells and clusters were identified in 29 (48%) and 14 (25%) of the samples, respectively. Two cells were identified in one of three non-malignant cases. Clusters were found exclusively in malignant cases. The occurrence of CK + cells or clusters was not associated with any of the evaluated clinicopathological factors, including surgical technique and tumor budding. Moreover, the occurrence of CK + cells or clusters had no influence on the cancer-specific survival [75 mo (CI: 61; 88) vs 83 mo (CI: 72; 95) and 80 mo (CI: 63; 98) vs 79 mo (CI: 69; 89), respectively]. Conclusion: CK + cells and showed neither prognostic significance nor an association with tumor budding. It is very likely that CK18-staining is not specific enough to identify the relevant cells. Competing Interests: Conflict-of-interest statement: All authors declare that no conflicting interests (including but not limited to commercial, personal, political, intellectual or religious interests) exist.
Databáze:	MEDLINE
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