Physico-chemical properties based differential toxicity of graphene oxide/reduced graphene oxide in human lung cells mediated through oxidative stress.

Autor: Mittal S; Academy of Scientific and Innovative Research (AcSIR), CSIR - IITR Campus, Lucknow, India.; Nanomaterials Toxicology Laboratory, Nanotherapeutics and Nanomaterial Toxicology Group, CSIR - Indian Institute of Toxicology Research (CSIR - IITR), Vishvigyan Bhawan, 31, Mahatma Gandhi Marg, PO Box - 80, Lucknow, Uttar Pradesh - 226001, India., Kumar V; CSIR - National Physical Laboratory (CSIR-NPL), New Delhi - 110012, India., Dhiman N; Academy of Scientific and Innovative Research (AcSIR), CSIR - IITR Campus, Lucknow, India.; Water Analysis Laboratory, Nanotherapeutics and Nanomaterial Toxicology Group, CSIR - Indian Institute of Toxicology Research (CSIR - IITR), Vishvigyan Bhawan, 31, Mahatma Gandhi Marg, PO Box - 80, Lucknow, Uttar Pradesh - 226001, India., Chauhan LK; Electron Microscopy Laboratory, CSIR - Indian Institute of Toxicology Research (CSIR - IITR), Vishvigyan Bhawan, 31, Mahatma Gandhi Marg, PO Box - 80, Lucknow, Uttar Pradesh - 226001, India., Pasricha R; CSIR - National Physical Laboratory (CSIR-NPL), New Delhi - 110012, India., Pandey AK; Academy of Scientific and Innovative Research (AcSIR), CSIR - IITR Campus, Lucknow, India.; Nanomaterials Toxicology Laboratory, Nanotherapeutics and Nanomaterial Toxicology Group, CSIR - Indian Institute of Toxicology Research (CSIR - IITR), Vishvigyan Bhawan, 31, Mahatma Gandhi Marg, PO Box - 80, Lucknow, Uttar Pradesh - 226001, India.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2016 Dec 21; Vol. 6, pp. 39548. Date of Electronic Publication: 2016 Dec 21.
DOI: 10.1038/srep39548
Abstrakt: Graphene derivatives (GD) are currently being evaluated for technological and biomedical applications owing to their unique physico-chemical properties over other carbon allotrope such as carbon nanotubes (CNTs). But, the possible association of their properties with underlying in vitro effects have not fully examined. Here, we assessed the comparative interaction of three GD - graphene oxide (GO), thermally reduced GO (TRGO) and chemically reduced GO (CRGO), which significantly differ in their lateral size and functional groups density, with phenotypically different human lung cells; bronchial epithelial cells (BEAS-2B) and alveolar epithelial cells (A549). The cellular studies demonstrate that GD significantly ineternalize and induce oxidative stress mediated cytotoxicity in both cells. The toxicity intensity was in line with the reduced lateral size and increased functional groups revealed more toxicity potential of TRGO and GO respectively. Further, A549 cells showed more susceptibility than BEAS-2B which reflected cell type dependent differential cellular response. Molecular studies revealed that GD induced differential cell death mechanism which was efficiently prevented by their respective inhibitors. This is prior study to the best of our knowledge involving TRGO for its safety evaluation which provided invaluable information and new opportunities for GD based biomedical applications.
Databáze: MEDLINE
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