Transcriptional signatures of somatic neoblasts and germline cells in Macrostomum lignano .

Autor:	Grudniewska M; European Research Institute for the Biology of Ageing, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands., Mouton S; European Research Institute for the Biology of Ageing, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.; Hubrecht Institute-KNAW, Utrecht, The Netherlands.; University Medical Centre Utrecht, Utrecht, The Netherlands., Simanov D; European Research Institute for the Biology of Ageing, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.; Hubrecht Institute-KNAW, Utrecht, The Netherlands.; University Medical Centre Utrecht, Utrecht, The Netherlands., Beltman F; European Research Institute for the Biology of Ageing, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands., Grelling M; European Research Institute for the Biology of Ageing, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands., de Mulder K; Hubrecht Institute-KNAW, Utrecht, The Netherlands.; University Medical Centre Utrecht, Utrecht, The Netherlands., Arindrarto W; Hubrecht Institute-KNAW, Utrecht, The Netherlands.; University Medical Centre Utrecht, Utrecht, The Netherlands., Weissert PM; European Research Institute for the Biology of Ageing, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands., van der Elst S; Hubrecht Institute-KNAW, Utrecht, The Netherlands.; University Medical Centre Utrecht, Utrecht, The Netherlands., Berezikov E; European Research Institute for the Biology of Ageing, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.; Hubrecht Institute-KNAW, Utrecht, The Netherlands.; University Medical Centre Utrecht, Utrecht, The Netherlands.
Jazyk:	angličtina
Zdroj:	ELife [Elife] 2016 Dec 20; Vol. 5. Date of Electronic Publication: 2016 Dec 20.
DOI:	10.7554/eLife.20607
Abstrakt:	The regeneration-capable flatworm Macrostomum lignano is a powerful model organism to study the biology of stem cells in vivo. As a flatworm amenable to transgenesis, it complements the historically used planarian flatworm models, such as Schmidtea mediterranea . However, information on the transcriptome and markers of stem cells in M. lignano is limited. We generated a de novo transcriptome assembly and performed the first comprehensive characterization of gene expression in the proliferating cells of M. lignano , represented by somatic stem cells, called neoblasts, and germline cells. Knockdown of a selected set of neoblast genes, including Mlig-ddx39 , Mlig-rrm1 , Mlig-rpa3 , Mlig-cdk1 , and Mlig-h2a , confirmed their crucial role for the functionality of somatic neoblasts during homeostasis and regeneration. The generated M. lignano transcriptome assembly and gene expression signatures of somatic neoblasts and germline cells will be a valuable resource for future molecular studies in M. lignano . Competing Interests: The authors declare that no competing interests exist.
Databáze:	MEDLINE
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