Simultaneous detection of sulfoxaflor and its metabolites, X11719474 and X11721061, in lettuce using a modified QuEChERS extraction method and liquid chromatography-tandem mass spectrometry.

Autor: Kim SW; Biotechnology Research Institute, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, Republic of Korea., Rahman MM; Biotechnology Research Institute, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, Republic of Korea., Abd El-Aty AM; Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea.; Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt., Kabir MH; Biotechnology Research Institute, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, Republic of Korea., Na TW; Jeonnam Bioindustry Foundation, Biocontrol Research Center Immyeon, Gokseonggun, Jellanamdo, Republic of Korea., Choi JH; Biotechnology Research Institute, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, Republic of Korea., Shin HC; Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea., Shim JH; Biotechnology Research Institute, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, Republic of Korea.
Jazyk: angličtina
Zdroj: Biomedical chromatography : BMC [Biomed Chromatogr] 2017 Jun; Vol. 31 (6). Date of Electronic Publication: 2016 Dec 19.
DOI: 10.1002/bmc.3885
Abstrakt: An analytical method has been developed to quantify the residual levels of sulfoxaflor and its metabolites (X11719474 and X11721061) in/on cultivated lettuce grown under greenhouse conditions. Samples were extracted and purified using a quick, easy, cheap, effective, rugged, and safe 'QuEChERS' method (original version) following systematic method optimization and were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Good linearity with coefficient of determination ≥0.9930 was obtained and the limits of detection (LOD) and quantification (LOQ) were in the ranges of 0.003-0.006 and 0.01-0.02 mg/kg, respectively. The recovery rates of both the parent compound and its metabolites (fortified at 10 and 50× the LOQ) estimated from six replicates ranged between 81.9 and 115.5% with a relative standard deviation <12%. The validated method was applied to field-incurred samples (collected over 7 days) sprayed once or twice with a water-dispersible granule formulation. Notably, a substantial reduction in rate was observed after 3 days and the half-life was short, only 1.5 days. The developed method is simple and versatile and can be used for various leafy vegetables.
(Copyright © 2016 John Wiley & Sons, Ltd.)
Databáze: MEDLINE
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