Highlighting Astyanax Species Diversity through DNA Barcoding.

Autor: Rossini BC; Laboratório de Biologia e Genética de Peixes, Departamento de Morfologia, UNESP-Universidade Estadual Paulista, Botucatu, São Paulo, Brazil.; Instituto de Biotecnologia da UNESP, UNESP-Universidade Estadual Paulista, Botucatu, São Paulo, Brazil., Oliveira CA; Universidade Estadual de Maringá, Programa de Pós-Graduação em Ecologia de Ambientes Aquáticos Continentais, Maringá, Paraná, Brazil., Melo FA; Universidade Estadual do Piauí, Campus Alexandre Alves de Oliveira, Parnaíba, Piauí, Brazil., Bertaco VA; Museu de Ciências Naturais, Fundação Zoobotânica do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil., Astarloa JM; Grupo de Biotaxonomía Morfológica y Molecular de Peces, Instituto de Investigaciones Marinas y Costeras, Universidad Nacional de Mar del Plata. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina., Rosso JJ; Grupo de Biotaxonomía Morfológica y Molecular de Peces, Instituto de Investigaciones Marinas y Costeras, Universidad Nacional de Mar del Plata. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina., Foresti F; Laboratório de Biologia e Genética de Peixes, Departamento de Morfologia, UNESP-Universidade Estadual Paulista, Botucatu, São Paulo, Brazil., Oliveira C; Laboratório de Biologia e Genética de Peixes, Departamento de Morfologia, UNESP-Universidade Estadual Paulista, Botucatu, São Paulo, Brazil.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2016 Dec 19; Vol. 11 (12), pp. e0167203. Date of Electronic Publication: 2016 Dec 19 (Print Publication: 2016).
DOI: 10.1371/journal.pone.0167203
Abstrakt: DNA barcoding has been used extensively to solve taxonomic questions and identify new species. Neotropical fishes are found in a wide variety of shapes and sizes, with a large number of species yet to be described, many of which are very difficult to identify. Characidae is the most species-rich family of the Characiformes, and many of its genera are affected by taxonomic uncertainties, including the widely-distributed, species-rich genus Astyanax. In this study, we present an extensive analysis of Astyanax covering almost its entire area of occurrence, based on DNA barcoding. The use of different approaches (ABGD, GMYC and BIN) to the clustering of the sequences revealed ample consistency in the results obtained by the initial cutoff value of 2% divergence for putative species in the Neighbor-Joining analysis using the Kimura-2-parameter model. The results indicate the existence of five Astyanax lineages. Some groups, such as that composed by the trans-Andean forms, are mostly composed of well-defined species, and in others a number of nominal species are clustered together, hampering the delimitation of species, which in many cases proved impossible. The results confirm the extreme complexity of the systematics of the genus Astyanax and show that DNA barcoding can be an useful tool to address these complexes questions.
Competing Interests: We don't have competing interests.
Databáze: MEDLINE
Externí odkaz: