Influence of chlorpromazine on renal histology of rats submitted to ischemia and reperfusion injury.

Autor:	Foiatto JC; Fellow Master degree, Postgraduate Program in Surgery Principles, Medical Research Institute, Faculdade Evangélica do Paraná (FEPAR), Curitiba-PR, Brazil. Conception, design, intellectual and scientific content of the study, manuscript preparation., Czeczko NG; Associate Professor, Postgraduate Program in Principles of Surgery, FEPAR, Universidade Evangélica, Hospital de Curitiba, Medical Research Institute, Curitiba-PR, Brazil. Intellectual and scientific content of the study, interpretation of data, critical revision., Wietzikoski EG; Fellow Master degree, Postgraduate Program in Surgery Principles, Medical Research Institute, Faculdade Evangélica do Paraná (FEPAR), Curitiba-PR, Brazil. Conception, design, intellectual and scientific content of the study, manuscript preparation., Malafaia O; Associate Professor, Postgraduate Program in Principles of Surgery, FEPAR, Universidade Evangélica, Hospital de Curitiba, Medical Research Institute, Curitiba-PR, Brazil. Intellectual and scientific content of the study, interpretation of data, critical revision., Brenny T Filho; Master, Urology Department, Hospital São Vicente, Curitiba-PR, Brazil. Manuscript preparation., Oliveira AM; MD, Uro-oncology Department, Hospital São Vicente, Curitiba-PR, Brazil. Manuscript preparation., Gomes RP; PhD, Pathology Department, Universidade Federal do Paraná (UFPR), Curitiba-PR, Brazil. Histopathological examinations.
Jazyk:	angličtina
Zdroj:	Acta cirurgica brasileira [Acta Cir Bras] 2016 Nov; Vol. 31 (11), pp. 759-764.
DOI:	10.1590/S0102-865020160110000009
Abstrakt:	Purpose:: To analyze the influence of chlorpromazine on renal histology of rats submitted to ischemia and reperfusion injury. Methods:: Sixteen Wistar rats - split in two groups - have been used: control group, receiving 3 mg/kg isotonic saline solution through caudal vein, and, the chlorpromazine group, receiving 3 mg/kg-IV of such medication. The nephrectomy of the left kidney lower third was carried out; immediately, the test-drug was administrated. After 15 minutes of test-drug administration, the renal pedicle was clamped; in 60 minutes of ischemia it was released. After 24 hours of the renal reperfusion, the rats were, once more, anesthetized and submitted to total left nephrectomy, and, afterwards, to euthanasia. Histological findings regarding ischemia have been evaluated and compared between the groups. Results:: There was no statistical difference related to inferior renal pole histological analysis. Regarding 60-minute renal ischemia, chlorpromazine has statistically reduced the accrual of leucocytes within the vasa recta renis (p=0.036) and the congestion of peritubular capillaries (p=0.041). When conducting joint analysis of histological patterns, the control group showed a median score of 11 and chlorpromazine group of 5.5 (p=0.036). Conclusion:: Chlorpromazine significantly reduced the occurrence of secondary damage to ischemia and reperfusion process in the overall histological analysis.
Databáze:	MEDLINE
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