Molecular mechanisms that distinguish TFIID housekeeping from regulatable SAGA promoters.

Autor: de Jonge WJ; Molecular Cancer Research, University Medical Center Utrecht, Utrecht, The Netherlands.; Princess Máxima Center for Pediatric Oncology, Utrecht, The Netherlands., O'Duibhir E; Molecular Cancer Research, University Medical Center Utrecht, Utrecht, The Netherlands., Lijnzaad P; Molecular Cancer Research, University Medical Center Utrecht, Utrecht, The Netherlands.; Princess Máxima Center for Pediatric Oncology, Utrecht, The Netherlands., van Leenen D; Molecular Cancer Research, University Medical Center Utrecht, Utrecht, The Netherlands., Groot Koerkamp MJ; Molecular Cancer Research, University Medical Center Utrecht, Utrecht, The Netherlands.; Princess Máxima Center for Pediatric Oncology, Utrecht, The Netherlands., Kemmeren P; Molecular Cancer Research, University Medical Center Utrecht, Utrecht, The Netherlands.; Princess Máxima Center for Pediatric Oncology, Utrecht, The Netherlands., Holstege FC; Molecular Cancer Research, University Medical Center Utrecht, Utrecht, The Netherlands f.c.p.holstege@prinsesmaximacentrum.nl.; Princess Máxima Center for Pediatric Oncology, Utrecht, The Netherlands.
Jazyk: angličtina
Zdroj: The EMBO journal [EMBO J] 2017 Feb 01; Vol. 36 (3), pp. 274-290. Date of Electronic Publication: 2016 Dec 15.
DOI: 10.15252/embj.201695621
Abstrakt: An important distinction is frequently made between constitutively expressed housekeeping genes versus regulated genes. Although generally characterized by different DNA elements, chromatin architecture and cofactors, it is not known to what degree promoter classes strictly follow regulatability rules and which molecular mechanisms dictate such differences. We show that SAGA-dominated/TATA-box promoters are more responsive to changes in the amount of activator, even compared to TFIID/TATA-like promoters that depend on the same activator Hsf1. Regulatability is therefore an inherent property of promoter class. Further analyses show that SAGA/TATA-box promoters are more dynamic because TATA-binding protein recruitment through SAGA is susceptible to removal by Mot1. In addition, the nucleosome configuration upon activator depletion shifts on SAGA/TATA-box promoters and seems less amenable to preinitiation complex formation. The results explain the fundamental difference between housekeeping and regulatable genes, revealing an additional facet of combinatorial control: an activator can elicit a different response dependent on core promoter class.
(© 2016 The Authors. Published under the terms of the CC BY NC ND 4.0 license.)
Databáze: MEDLINE
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