Orthogonal Cysteine Protection Enables Homogeneous Multi-Drug Antibody-Drug Conjugates.

Autor: Levengood MR; Seattle Genetics, Inc., 21823 30thDrive SE, Bothell, WA, USA., Zhang X; Seattle Genetics, Inc., 21823 30thDrive SE, Bothell, WA, USA., Hunter JH; Seattle Genetics, Inc., 21823 30thDrive SE, Bothell, WA, USA., Emmerton KK; Seattle Genetics, Inc., 21823 30thDrive SE, Bothell, WA, USA., Miyamoto JB; Seattle Genetics, Inc., 21823 30thDrive SE, Bothell, WA, USA., Lewis TS; Seattle Genetics, Inc., 21823 30thDrive SE, Bothell, WA, USA., Senter PD; Seattle Genetics, Inc., 21823 30thDrive SE, Bothell, WA, USA.
Jazyk: angličtina
Zdroj: Angewandte Chemie (International ed. in English) [Angew Chem Int Ed Engl] 2017 Jan 16; Vol. 56 (3), pp. 733-737. Date of Electronic Publication: 2016 Dec 14.
DOI: 10.1002/anie.201608292
Abstrakt: A strategy for the preparation of homogeneous antibody-drug conjugates (ADCs) containing multiple payloads has been developed. This approach utilizes sequential unmasking of cysteine residues with orthogonal protection to enable site-specific conjugation of each drug. In addition, because the approach utilizes conjugation to native antibody cysteine residues, it is widely applicable and enables high drug loading for improved ADC potency. To highlight the benefits of ADC dual drug delivery, this strategy was applied to the preparation of ADCs containing two classes of auristatin drug-linkers that have differing physiochemical properties and exert complementary anti-cancer activities. Dual-auristatin ADCs imparted activity in cell line and xenograft models that are refractory to ADCs comprised of the individual auristatin components. This work presents a facile method for construction of potent dual-drug ADCs and demonstrates how delivery of multiple cytotoxic warheads can lead to improved ADC activities. Lastly, we anticipate that the conditions utilized herein for orthogonal cysteine unmasking are not restricted to ADCs and can be broadly utilized for site-specific protein modification.
(© 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.)
Databáze: MEDLINE
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