Calmodulin Regulated Spectrin Associated Protein 1 mRNA is Directly Regulated by miR-126 in Primary Human Osteoblasts.

Autor: Strassburg S; Departments of Plastic and Hand Surgery, University of Freiburg Medical Center, Freiburg, 79106, Germany., Nabar N; Departments of Plastic and Hand Surgery, University of Freiburg Medical Center, Freiburg, 79106, Germany., Lampert F; Departments of Plastic and Hand Surgery, University of Freiburg Medical Center, Freiburg, 79106, Germany., Goerke SM; Department of Radiology, Ortenau Klinikum Offenburg-Gengenbach, Offenburg, Germany., Pfeifer D; Department of Hematology and Oncology, Freiburg University Medical Center, Freiburg, 79106, Germany., Finkenzeller G; Departments of Plastic and Hand Surgery, University of Freiburg Medical Center, Freiburg, 79106, Germany., Stark GB; Departments of Plastic and Hand Surgery, University of Freiburg Medical Center, Freiburg, 79106, Germany., Simunovic F; Departments of Plastic and Hand Surgery, University of Freiburg Medical Center, Freiburg, 79106, Germany.
Jazyk: angličtina
Zdroj: Journal of cellular biochemistry [J Cell Biochem] 2017 Jul; Vol. 118 (7), pp. 1756-1763. Date of Electronic Publication: 2017 Jan 05.
DOI: 10.1002/jcb.25838
Abstrakt: Vascularization is essential for bone development, fracture healing, and bone tissue engineering. We have previously described that coculture of primary human osteoblasts (hOBs) and human umbilical vein endothelial cells (HUVECs) improves differentiation of both cell types. Investigating the role of microRNAs (miRNAs) in this system, we found that miR-126 is highly upregulated in hOBs following coculturing with HUVECs. In this study we performed miR-126 gain-of-function and loss-of-function experiments in hOBs followed by microarray analysis in order to identify targets of miR-126. The transcript cluster IDs were sieved by applying cut-off criteria and by selecting transcripts which were upregulated following miR-126 downregulation and vice versa. The calmodulin regulated spectrin associated protein 1 (CAMSAP1) mRNA was confirmed to be differentially regulated by miR-126. Using the luciferase reporter assay it was demonstrated that CAMSAP1 is directly targeted by miR-126. In this study, we show that miR-126 and CAMSAP1 directly interact in hOBs. This finding has potential implications for tissue engineering applications. J. Cell. Biochem. 118: 1756-1763, 2017. © 2016 Wiley Periodicals, Inc.
(© 2016 Wiley Periodicals, Inc.)
Databáze: MEDLINE
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