| Autor: |
de Moraes GMD; Rede Nordeste de Biotecnologia (RENORBIO), Universidade Estadual do Ceará, Fortaleza, CE, Brazil., de Abreu LR; Programa de Pós Graduação em Biotecnologia, Universidade Federal do Ceará, Sobral, CE, Brazil., do Egito AS; Embrapa Caprinos e Ovinos, Sobral, CE, Brazil., Salles HO; Embrapa Caprinos e Ovinos, Sobral, CE, Brazil., da Silva LMF; Embrapa Caprinos e Ovinos, Sobral, CE, Brazil., Nero LA; Departamento de Veterinária, Universidade Federal de Viçosa, Viçosa, MG, Brazil., Todorov SD; Departamento de Veterinária, Universidade Federal de Viçosa, Viçosa, MG, Brazil., Dos Santos KMO; Embrapa Agroindústria de Alimentos, Rio de Janeiro, RJ, Brazil. karina.dos-santos@embrapa.br. |
| Abstrakt: |
The search for probiotic candidates among lactic acid bacteria (LAB) isolated from food may uncover new strains with promising health and technological properties. Lactobacillus mucosae strains attracted recent research attention due to their ability to adhere to intestinal mucus and to inhibit pathogens in the gastrointestinal tract, both related to a probiotic potential. Properties of interest and safety aspects of three Lb. mucosae strains (CNPC006, CNPC007, and CNPC009) isolated from goat milk were investigated employing in vitro tests. The presence of genetic factors related to bile salt hydrolase production (bsh), intestinal adhesion properties (msa, map, mub, and ef-tu), virulence, and biogenic amine production were also verified. All strains exhibited the target map, mub, and ef-tu sequences; the msa gene was detected in CNPC006 and CNPC007 strains. Some of the searched sequences for virulence factors were detected, especially in the CNPC009 strain; all strains carried the hyl gene, related to the production of hyaluronidase. Lb. mucosae CNPC007 exhibited a high survival rate in simulated gastric and enteric conditions. Besides, all strains exhibited the bsh sequence, and CNPC006 and CNPC007 were able to deconjugate salts of glycodeoxycholic acid (GDC). Regarding technological properties for dairy product applications, a relatively higher milk acidification and clotting capacity, diacetyl production, and proteolytic activity were registered for CNPC007 in comparison to the other strains. Collectively, the results aim at Lb. mucosae CNPC007 as a promising probiotic candidate for application in dairy products, deserving further studies to confirm and explore its potential. |
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